Modulation of Alveolar Macrophage Activity by Eugenol Attenuates Cigarette-Smoke-Induced Acute Lung Injury in Mice

Maria Clara Barbosa-de-Oliveira; Paolo Oliveira-Melo; Marcos Henrique Gonçalves da Silva; Flávio Santos da Silva; Felipe Andrade Carvalho da Silva; Bruno Vinicios Silva de Araujo; Moacir Franco de Oliveira; Aristides Tadeu Correia; Sidnei Miyoshi Sakamoto; Samuel Santos Valença; Manuella Lanzetti; Martina Schmidt; Emanuel Kennedy-Feitosa

doi:10.3390/antiox12061258

Modulation of Alveolar Macrophage Activity by Eugenol Attenuates Cigarette-Smoke-Induced Acute Lung Injury in Mice

Antioxidants (Basel). 2023 Jun 11;12(6):1258. doi: 10.3390/antiox12061258.

Authors

Maria Clara Barbosa-de-Oliveira¹, Paolo Oliveira-Melo², Marcos Henrique Gonçalves da Silva³, Flávio Santos da Silva¹, Felipe Andrade Carvalho da Silva¹, Bruno Vinicios Silva de Araujo¹, Moacir Franco de Oliveira⁴, Aristides Tadeu Correia², Sidnei Miyoshi Sakamoto¹, Samuel Santos Valença⁵, Manuella Lanzetti⁵, Martina Schmidt^{6

7}, Emanuel Kennedy-Feitosa¹

Affiliations

¹ Morphophysiopharmacology Laboratory, Department of Health Sciences, Federal University of the Semi-Arid Region, Mossoró 59625-900, Brazil.
² Laboratório de Pesquisa em Cirurgia Torácica, Faculdade de Medicina HCFMUSP, Universidade de Sao Paulo, Sao Paulo 05508-220, Brazil.
³ Department of Clinical Medicine, Federal Fluminense University (UFF), Niterói 24000-000, Brazil.
⁴ Department of Animal Science, Federal University of the Semi-Arid Region, Mossoró 59625-900, Brazil.
⁵ Institute of Biomedical Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-590, Brazil.
⁶ Department of Molecular Pharmacology, University of Groningen, Antonius Deusinglaan 1, Building 3211, Room 406, 9713 AV Groningen, The Netherlands.
⁷ Groningen Research Institute of Asthma and COPD, GRIAC, University Medical Center Groningen, University of Groningen, 9713 AV Groningen, The Netherlands.

Abstract

This study investigates the role of eugenol (EUG) on CS-induced acute lung injury (ALI) and how this compound is able to modulate macrophage activity. C57BL/6 mice were exposed to 12 cigarettes/day/5days and treated 15 min/day/5days with EUG. Rat alveolar macrophages (RAMs) were exposed to CSE (5%) and treated with EUG. In vivo, EUG reduced morphological changes inflammatory cells, oxidative stress markers, while, in vitro, it induced balance in the oxidative stress and reduced the pro-inflammatory cytokine release while increasing the anti-inflammatory one. These results suggest that eugenol reduced CS-induced ALI and acted as a modulator of macrophage activity.

Keywords: cigarette; eugenol; macrophages; oxidative stress; pulmonary inflammation.

Grants and funding

PPP 25/2018 - 23091.014011/2018-05/Universidade Federal Rural do Semi-Árido