Genetic Dissection of Spike Productivity Traits in the Siberian Collection of Spring Barley

Irina V Rozanova; Yuriy N Grigoriev; Vadim M Efimov; Alexander V Igoshin; Elena K Khlestkina

doi:10.3390/biom13060909

Genetic Dissection of Spike Productivity Traits in the Siberian Collection of Spring Barley

Biomolecules. 2023 May 30;13(6):909. doi: 10.3390/biom13060909.

Authors

Irina V Rozanova^{1

2}, Yuriy N Grigoriev³, Vadim M Efimov², Alexander V Igoshin², Elena K Khlestkina^{1

2}

Affiliations

¹ N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources (VIR), 190000 St. Petersburg, Russia.
² Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentjeva Ave. 10, 630090 Novosibirsk, Russia.
³ Siberian Research Institute of Plant Cultivation and Breeding-Branch of Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Krasnoobsk, 630501 Novosibirsk, Russia.

Abstract

Barley (Hordeum vulgare L.) is one of the most commonly cultivated cereals worldwide. Its local varieties can represent a valuable source of unique genetic variants useful for crop improvement. The aim of this study was to reveal loci contributing to spike productivity traits in Siberian spring barley and to develop diagnostic DNA markers for marker-assisted breeding programs. For this purpose we conducted a genome-wide association study using a panel of 94 barley varieties. In total, 64 SNPs significantly associated with productivity traits were revealed. Twenty-three SNP markers were validated by genotyping in an independent sample set using competitive allele-specific PCR (KASP). Finally, fourteen markers associated with spike productivity traits on chromosomes 2H, 4H and 5H can be suggested for use in breeding programs.

Keywords: GWAS; KASP; PLS; SNP; barley; meta-analysis; row.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
Genome-Wide Association Study
Hordeum* / genetics
Phenotype
Plant Breeding
Polymorphism, Single Nucleotide / genetics
Quantitative Trait Loci

Grants and funding

The cultivation of the barley plants and phenotyping were supported by ICG project FWNR-2022-0017, and the statistical processing and interpretation were funded by VIR project 15N 0481-2022-0007. Analysis of candidate gene in the significant genome regions was supported by Russian Science Foundation Project No. 21-66-00012.