The Use of Phage Cocktail and Various Antibacterial Agents in Combination to Prevent the Emergence of Phage Resistance

Hoang Minh Duc; Yu Zhang; Son Minh Hoang; Yoshimitsu Masuda; Ken-Ichi Honjoh; Takahisa Miyamoto

doi:10.3390/antibiotics12061077

The Use of Phage Cocktail and Various Antibacterial Agents in Combination to Prevent the Emergence of Phage Resistance

Antibiotics (Basel). 2023 Jun 20;12(6):1077. doi: 10.3390/antibiotics12061077.

Authors

Hoang Minh Duc^{1

2}, Yu Zhang^{1

3}, Son Minh Hoang^{1

4}, Yoshimitsu Masuda¹, Ken-Ichi Honjoh¹, Takahisa Miyamoto¹

Affiliations

¹ Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
² Department of Veterinary Public Health, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 12400, Vietnam.
³ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
⁴ Department of Histology and Embryo, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 12400, Vietnam.

Abstract

Bacterial food poisoning cases due to Salmonella and E. coli O157:H7 have been linked with the consumption of a variety of food products, threatening public health around the world. This study describes the combined effects of a phage cocktail (STG2, SEG5, and PS5), EDTA, nisin, and polylysine against the bacterial cocktail consisting of S. typhimurium, S. enteritidis, and E. coli O157:H7. Overall, phage cocktail (alone or in combination with nisin or/and polylysine) not only showed great antibacterial effects against bacterial cocktail at different temperatures (4 °C, 24 °C, and 37 °C), but also totally inhibited the emergence of phage resistance during the incubation period. These results suggest that the combination of phages with nisin or/and polylysine has great potential to simultaneously control S. typhimurium, S. enteritidis, and E. coli O157:H7.

Keywords: EDTA; bacterial cocktail; bacteriophages; biocontrol; nisin; polylysine.

Grants and funding

This research received no external funding.