Melanin-concentrating hormone and orexin shape social affective behavior via action in the insular cortex of rat

Lucas Barretto-de-Souza; Shemar A Joseph; Francesca M Lynch; Alexandra J Ng; Carlos C Crestani; John P Christianson

doi:10.1007/s00213-023-06408-5

Melanin-concentrating hormone and orexin shape social affective behavior via action in the insular cortex of rat

Psychopharmacology (Berl). 2023 Jun 28. doi: 10.1007/s00213-023-06408-5. Online ahead of print.

Authors

Lucas Barretto-de-Souza^{1

2}, Shemar A Joseph², Francesca M Lynch², Alexandra J Ng², Carlos C Crestani¹, John P Christianson³

Affiliations

¹ Laboratory of Pharmacology, São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, SP, Brazil.
² Department of Psychology & Neuroscience, Boston College, Chestnut Hill, MA, 02467, USA.
³ Department of Psychology & Neuroscience, Boston College, Chestnut Hill, MA, 02467, USA. j.christianson@bc.edu.

PMID: 37369782
DOI: 10.1007/s00213-023-06408-5

Abstract

Rationale: In a social context, individuals are able to detect external information from others and coordinate behavioral responses according to the situation, a phenomenon called social decision-making. Social decision-making is multifaceted, influenced by emotional and motivational factors like stress, sickness, and hunger. However, the neurobiological basis for motivational state competition and interaction is not well known.

Objective: We investigated possible neural mechanisms through which internal states could shape social behavior in a social affective preference (SAP) test. In the SAP test, experimental rats given a choice to interact with naïve or stressed conspecifics exhibit an age-dependent preference to interact with stressed juvenile conspecifics, but avoid stressed adult conspecifics. First, we assessed the effect of food and water deprivation on SAP behavior. Behavior in the SAP test requires the insular cortex, which receives input from the ingestion-related peptides melanin-concentrating hormone (MCH) and orexin neurons of the lateral hypothalamus (LH). This study aimed to evaluate the role of LH and insular MCH and orexin in SAP test.

Methods: SAP tests were conducted in rats that were sated, food and water deprived or allowed 1 h of access to food and water after 14 h of deprivation (relieved condition). Separate cohorts of sated rats received cannula implants for microinjection of drugs to inhibit the LH or to block or stimulate MCH or orexin receptors in the insula prior to SAP tests or social interaction tests.

Results: Food and water deprivation prior to SAP tests with juvenile rats caused a shift in preference away from the stressed rat toward the naïve juveniles. Pharmacological inhibition of LH with muscimol (100 ng/side) abolished the preference for the juvenile-stressed conspecific, as well as the preference for the adult naïve conspecific. The blockade of MCH receptor 1or orexin receptors in the insular cortex with SNAP94847 (50 μM) or TCS1102 (1 μM), respectively, also abolished the preference for the stressed juvenile conspecific, but only the antagonism of orexin receptors was able to abolish the preference for the adult naïve conspecific. Microinjection of increasing doses (50 or 500 nM) of MCH or orexin-A in the insular cortex increased the interaction time in the one-on-one social interaction test with juvenile conspecifics; however, only the microinjection of orexin-A increased the interaction time with adult naïve conspecifics.

Conclusions: Taken together, these results suggest that lateral hypothalamus peptides shape the direction of social approach or avoidance via actions MCH and orexin neurotransmission in the insular cortex.

Keywords: Hunger; Insular cortex; Lateral hypothalamus; Melanin-concentrating hormone; Orexin; Social affective preference; Thirst.