Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle

Mona Al-Sharif; Mohamed Abdo; Omnia El Shabrawy; Eman M Abu El-Naga; Liana Fericean; Ioan Banatean-Dunea; Ahmed Ateya

doi:10.3390/vetsci10060370

Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle

Vet Sci. 2023 May 23;10(6):370. doi: 10.3390/vetsci10060370.

Authors

Mona Al-Sharif¹, Mohamed Abdo^{2

3}, Omnia El Shabrawy⁴, Eman M Abu El-Naga⁵, Liana Fericean⁶, Ioan Banatean-Dunea⁶, Ahmed Ateya⁷

Affiliations

¹ Department of Biology, College of Science, University of Jeddah, Jeddah 21589, Saudi Arabia.
² Department of Animal Histology and Anatomy, School of Veterinary Medicine, Badr University in Cairo (BUC), Cairo 11829, Egypt.
³ Department of Anatomy and Embryology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Egypt.
⁴ Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Menofia University, Menofia 32951, Egypt.
⁵ Department of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt.
⁶ Department of Biology and Plant Protection, Faculty of Agricultural Sciences, University of Life Sciences King Michael I, 300645 Timisoara, Romania.
⁷ Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.

Abstract

This study looked at genetic polymorphisms and transcript levels of immune, antioxidant, and erythritol-related markers for postparturient endometritis prediction and tracking in Holstein dairy cows. One hundred and thirty female dairy cows (65 endometritis affected and 65 apparently healthy) were used. Nucleotide sequence variations between healthy and endometritis-affected cows were revealed using PCR-DNA sequencing for immune (TLR4, TLR7, TNF-α, IL10, NCF4, and LITAF), antioxidant (ATOX1, GST, and OXSR1), and erythritol-related (TKT, RPIA, and AMPD1) genes. Chi-square investigation exposed a noteworthy variance amongst cow groups with and without endometritis in likelihood of dispersal of all distinguished nucleotide variants (p < 0.05). The IL10, ATOX1, and GST genes were expressed at substantially lower levels in endometritis-affected cows. Gene expression levels were considerably higher in endometritis-affected cows than in resistant ones for the genes TLR4, TLR7, TNF-α, NCF4, LITAF, OXSR1, TKT, RPIA, and AMPD1. The sort of marker and vulnerability or resistance to endometritis had a significant impact on the transcript levels of the studied indicators. The outcomes might confirm the importance of nucleotide variants along with gene expression patterns as markers of postparturient endometritis susceptibility/resistance and provide a workable control plan for Holstein dairy cows.

Keywords: Holstein cattle; genetic polymorphisms; postparturient endometritis; transcript level.

Grants and funding

The Research Institute for Biosecurity and Bioengineering from Timisoara and the University of Life Sciences “King Mihai I” from Timisoara collaborated on the project 6PFE that resulted in the publication of this article.