Background: Plants are harmed by parasitic organisms, and toxic poisons are created. Phytopathogenic fungi create toxins that can severely harm plants' basic physiological functioning.
Objective: Investigation of antifungal impact of various fractions of methanol extract of Artemisia herba-alba to Aspergillus niger as a plant pathogen.
Methods: Artemisia herba-alba extract was purified using column chromatography, giving various antifungal fractions tested versus A. niger.
Results: The 6th fraction give the highest inhibition zone with a diameter of 5.4 cm and MIC 125.02 ± 4.9 μg/ml, which was identified using Mass spectroscopy, 1HNMR, Elemental analysis as well as IR testing, revealing the chemical formula of the purified fraction. Ultrastructure alteration of treated A. niger was examined versus control using the transmission electron microscope. Purified fraction has tested versus normal cell line with minimal cytotoxicity.
Conclusion: These results revealed the possibility of using Artemisia herba-alba methanol extract as a promising antifungal versus phytopathogenic fungi, especially A. niger after more verification of results.
Keywords: Artemisia herba-alba; Aspergillus niger; antifungal action.; antifungal activity; cytotoxicity; pathogen.
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