In recent years, magnetic nanoparticle sensor technologies have attracted considerable interest in the point-of-care-testing (POCT) field, especially in lateral flow immunoassays (LFIAs). Although the visual signal of magnetic nanoparticles is reduced during an inspection, it can be compensated for by magnetic induction, and detection results can be quantified by magnetic sensors. Sensors that use magnetic nanoparticles (MNPs) as markers can overcome the high background noise of complex samples. In this study, MNP signal detection strategies are described from the perspectives of magnetoresistance, magnetic flux, frequency mixing technology, and magnetic permeability, and the principles and development of each technology are introduced in detail. Typical applications of magnetic nanoparticle sensor technologies are introduced. By describing the advantages and limitations of different sensing strategies, we highlight the development and improvement directions of different sensing strategies. In general, the future development of magnetic nanoparticle sensor technologies will be toward intelligent, convenient, and mobile high-performance detection equipment.