Approaches to Measuring Reductive and Oxidative Events in Phagosomes

Shranjit S Lail; Dale R Balce; Johnathan Canton; Robin M Yates

doi:10.1007/978-1-0716-3338-0_10

Approaches to Measuring Reductive and Oxidative Events in Phagosomes

Methods Mol Biol. 2023:2692:139-152. doi: 10.1007/978-1-0716-3338-0_10.

Authors

Shranjit S Lail¹, Dale R Balce², Johnathan Canton^{2

3}, Robin M Yates^{4

5

6}

Affiliations

¹ Department of Medical Science, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
² Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.
³ Snyder Institute of Chronic Disease, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
⁴ Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada. rmyates@ucalgary.ca.
⁵ Snyder Institute of Chronic Disease, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. rmyates@ucalgary.ca.
⁶ Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. rmyates@ucalgary.ca.

PMID: 37365466
DOI: 10.1007/978-1-0716-3338-0_10

Abstract

The phagosome is a redox-active organelle. Numerous reductive and oxidative systems play both direct and indirect roles in phagosomal function. With the advent of newer methodologies to study these redox events in live cells, the details of how redox conditions change within the maturing phagosome, how they are regulated, and how they influence other phagosomal functions can be investigated. In this chapter, we detail phagosome-specific, fluorescence-based assays that measure disulfide reduction and the production of reactive oxygen species in live phagocytes such as macrophages and dendritic cells, in real time.

Keywords: Disulfide reduction; Lysosome; Oxidation; Phagosome; Redox.

MeSH terms

Macrophages*
Oxidation-Reduction
Oxidative Stress
Phagosomes* / metabolism
Reactive Oxygen Species / metabolism

Substances

Reactive Oxygen Species