Characterization of intratumoral tertiary lymphoid structures in pancreatic ductal adenocarcinoma: cellular properties and prognostic significance

Xuan Zou; Xuan Lin; He Cheng; Yusheng Chen; Ruijie Wang; Mingjian Ma; Yu Liu; Zhengjie Dai; Yesboli Tasiheng; Yu Yan; Qinqin Hou; Fei Ding; Huan Chen; Xianjun Yu; Xu Wang; Chen Liu

doi:10.1136/jitc-2023-006698

Characterization of intratumoral tertiary lymphoid structures in pancreatic ductal adenocarcinoma: cellular properties and prognostic significance

J Immunother Cancer. 2023 Jun;11(6):e006698. doi: 10.1136/jitc-2023-006698.

Authors

Xuan Zou^#^{1

2

3

4}, Xuan Lin^#^{1

2

3

4}, He Cheng^#^{1

2

3

4}, Yusheng Chen^#^{1

2

3

4}, Ruijie Wang^{1

2

3

4}, Mingjian Ma^{1

2

3

4}, Yu Liu^{1

2

3

4}, Zhengjie Dai^{1

2

3

4}, Yesboli Tasiheng^{1

2

3

4}, Yu Yan^{1

2

3

4}, Qinqin Hou⁵, Fei Ding⁵, Huan Chen⁵, Xianjun Yu^{6

2

3

4}, Xu Wang^{6

2

3

4

7}, Chen Liu^{6

2

3

4}

Affiliations

¹ Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.
² Department of Oncology, Shanghai Medical College, Shanghai, People's Republic of China.
³ Shanghai Pancreatic Cancer Institute, Shanghai, People's Republic of China.
⁴ Pancreatic Cancer Institute, Fudan University, Shanghai, People's Republic of China.
⁵ Department of Pathology Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.
⁶ Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China liuchen@fudanpci.org wangxu2013@fudan.edu.cn yuxianjun@fudanpci.org.
⁷ Shanghai Key Laboratory of Radiation Oncology, Cancer Research Institute, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, People's Republic of China.

^# Contributed equally.

Abstract

Background: Tumor-associated tertiary lymphoid structures (TLSs) are functional immune-responsive niches that are not fully understood in pancreatic ductal adenocarcinoma (PDAC).

Methods: Fluorescent multiplex immunohistochemistry was performed on sequential sections of surgically resected tumor tissues from 380 PDAC patients without preoperative treatment (surgery alone (SA)) and 136 patients pretreated with neoadjuvant treatment (NAT). Multispectral images were processed via machine learning and image processing platforms, inForm V.2.4 and HALO V.3.2; TLS regions were segmented, and the cells were identified and quantified. The cellular composition and immunological properties of TLSs and their adjacent tissues in PDAC were scored and compared, and their association with prognosis was further examined.

Results: Intratumoral TLSs were identified in 21.1% (80/380) of patients in the SA group and 15.4% (21/136) of patients in the NAT group. In the SA group, the presence of intratumoral TLSs was significantly associated with improved overall survival (OS) and progression-free survival. The existence of intratumoral TLSs was correlated with elevated levels of infiltrating CD8+T, CD4+T, B cells and activated immune cells in adjacent tissues. A nomogram model was generated with TLS presence as a variable, which successfully predicted PDAC patient OS in an external validation cohort (n=123). In the NAT group, samples exhibited a lower proportion of B cells and a higher proportion of regulatory T cells within intratumoral TLSs. Additionally, these TLSs were smaller in size, with a lower overall maturation level and reduced immune cell activation, and the prognostic value of TLS presence was insignificant in the NAT cohort.

Conclusion: Our study systematically revealed the cellular properties and prognostic values of intratumoral TLSs in PDAC and described the potential impact of NAT on TLS development and function.

Keywords: Gastrointestinal Neoplasms; Tumor Biomarkers; Tumor Microenvironment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Pancreatic Ductal* / pathology
Humans
Pancreatic Neoplasms* / pathology
Prognosis
Tertiary Lymphoid Structures*