Comparison of structure and neuroprotective ability of low molecular weight galactomannans from Sesbania cannabina obtained by different extraction technologies

Bowen Yan; Junping Deng; Jie Gu; Yuheng Tao; Caoxing Huang; Chenhuan Lai; Qiang Yong

doi:10.1016/j.foodchem.2023.136642

Comparison of structure and neuroprotective ability of low molecular weight galactomannans from Sesbania cannabina obtained by different extraction technologies

Food Chem. 2023 Nov 30:427:136642. doi: 10.1016/j.foodchem.2023.136642. Epub 2023 Jun 19.

Authors

Bowen Yan¹, Junping Deng², Jie Gu³, Yuheng Tao⁴, Caoxing Huang⁵, Chenhuan Lai⁵, Qiang Yong⁶

Affiliations

¹ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China.
² Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
³ Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
⁴ School of Pharmacy, School of Biology and Food Engineering, Changzhou University, Changzhou 213164, China.
⁵ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
⁶ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. Electronic address: swhx@njfu.com.cn.

PMID: 37364317
DOI: 10.1016/j.foodchem.2023.136642

Abstract

Low-molecular-weight-galactomannan (LMW-GM) is an edible polysaccharide with various biological activities. However, it is used in the field of neuroprotection. In this study, two types of LMW-GMs from Sesbania cannabina were obtained by gluconic acid extraction (GA-LMW-GM) and enzymatic hydrolysis (GMOS). The structure of GA-LMW-GM and GMOS were identified using different nuclear magnetic resonance (NMR) techniques. The antioxidant and neuroprotective activities of GA-LMW-GM and GMOS were evaluated in vitro/vivo. The results showed that both GA-LMW-GM and GMOS possess good free radicals scavenging ability in vitro with IC₅₀ values of 1.9 mg/mL and 4.9 mg/mL for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals 2.8 mg/mL and 4.4 mg/mL for O₂^•- radicals, respectively. However, GA-LMW-GM was more effective at scavenging reactive oxygen species (ROS) in vivo and protecting the fundamental growth (with a recovery capability of 62.5%) and locomotor functions (with recovery capability of 193.7%) of zebrafish with neurological damage induced by Bisphenol AF.

Keywords: Galactomannans; Gluconic acid; Molecular weight; Neuroprotection; Oxidative stress.

Publication types

Comparative Study

MeSH terms

Animals
Antioxidants / pharmacology
Molecular Weight
Neuroprotection
Sesbania* / chemistry
Zebrafish

Substances

Antioxidants
galactomannan