A comparative study of iron nanoflower and nanocube in terms of antibacterial properties

Ozan Eskikaya; Sadin Özdemir; Serpil Gonca; Nadir Dizge; Deepanraj Balakrishnan; Feroz Shaik; Natarajan Senthilkumar

doi:10.1007/s13204-023-02822-5

A comparative study of iron nanoflower and nanocube in terms of antibacterial properties

Appl Nanosci. 2023 Apr 5:1-13. doi: 10.1007/s13204-023-02822-5. Online ahead of print.

Authors

Ozan Eskikaya¹, Sadin Özdemir², Serpil Gonca³, Nadir Dizge¹, Deepanraj Balakrishnan⁴, Feroz Shaik⁴, Natarajan Senthilkumar⁵

Affiliations

¹ Department of Environmental Engineering, Mersin University, 33343 Mersin, Turkey.
² Technical Science Vocational School, Mersin University, Yenisehir, 33343 Mersin, Turkey.
³ Faculty of Pharmacy, University of Mersin, Turkey, Yenisehir, 33343 Mersin, Turkey.
⁴ College of Engineering, Prince Mohammad Bin Fahd University, Al Khobar, 31952 Saudi Arabia.
⁵ Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Chennai, 602105 India.

Abstract

It is known that heavy metal containing nanomaterials can easily prevent the formation of microbial cultures. The emergence of new generation epidemic diseases in the last 2 years has increased the importance of both personal and environmental hygiene. For this reason, in addition to preventing the spread of diseases, studies on alternative disinfectant substances are also carried out. In this study, the antibacterial activity of nanoflower and nanocube, which are easily synthesized and nanoparticle species containing iron, were compared. The antioxidant abilities of new synthesized NF@FeO(OH) and NC@α-Fe₂O₃ were tested by DPPH scavenging activity assay. The highest DPPH inhibition was achieved with NC@α-Fe₂O₃ as 71.30% at 200 mg/L. NF@FeO(OH) and NC@α-Fe₂O₃ demonstrated excellent DNA cleavage ability. The antimicrobial capabilities of NF@FeO(OH) and NC@α-Fe₂O₃ were analyzed with micro dilution procedure. In 500 mg/L, the antimicrobial activity was 100%. In addition to these, the biofilm inhibition of NF@FeO(OH) and NC@α-Fe₂O₃ were investigated against S. aureus and P. aeruginosa and it was found that they showed significant antibiofilm inhibition. It is suggested that additional studies can be continued to be developed and used as an antibacterial according to the results of the nanoparticles after various toxicological test systems.

Supplementary information: The online version contains supplementary material available at 10.1007/s13204-023-02822-5.

Keywords: Antimicrobial activity; Biofilm inhibition; DNA cleavage; Microbial cell viability; Nanocube; Nanoflower.

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