Effects of sources and levels of dietary supplementary manganese on growing yak's in vitro rumen fermentation

Huizhen Lu; Pengpeng Liu; Shujie Liu; Xinsheng Zhao; Binqiang Bai; Jianbo Cheng; Zijun Zhang; Cai Sun; Lizhuang Hao; Yanfeng Xue

doi:10.3389/fvets.2023.1175894

Effects of sources and levels of dietary supplementary manganese on growing yak's in vitro rumen fermentation

Front Vet Sci. 2023 Jun 9:10:1175894. doi: 10.3389/fvets.2023.1175894. eCollection 2023.

Authors

Huizhen Lu^{1

2

3}, Pengpeng Liu¹, Shujie Liu⁴, Xinsheng Zhao⁴, Binqiang Bai⁴, Jianbo Cheng¹, Zijun Zhang¹, Cai Sun³, Lizhuang Hao⁴, Yanfeng Xue^{1

3

4}

Affiliations

¹ Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
² Biotechnology Center, Anhui Agricultural University, Hefei, China.
³ Qinghai Pure Yak Biotechnology Co., LTD., Xining, China.
⁴ State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Qinghai Plateau Yak Research Center, Qinghai Academy of Science and Veterinary Medicine, Qinghai University, Xining, China.

Abstract

Introduction: Manganese (Mn) is an essential trace element for livestock, but little is known about the optimal Mn source and level for yak.

Methods: To improve yak's feeding standards, a 48-h in vitro study was designed to examine the effect of supplementary Mn sources including Mn sulfate (MnSO₄), Mn chloride (MnCl₂), and Mn methionine (Met-Mn) at five Mn levels, namely 35 mg/kg, 40 mg/kg, 50 mg/kg, 60 mg/kg, and 70 mg/kg dry matter (includes Mn in substrates), on yak's rumen fermentation.

Results: Results showed that Met-Mn groups showed higher acetate (p < 0.05), propionate, total volatile fatty acids (p < 0.05) levels, ammonia nitrogen concentration (p < 0.05), dry matter digestibility (DMD), and amylase activities (p < 0.05) compared to MnSO4 and MnCl2 groups. DMD (p < 0.05), amylase activities, and trypsin activities (p < 0.05) all increased firstly and then decreased with the increase of Mn level and reached high values at 40-50 mg/kg Mn levels. Cellulase activities showed high values (p < 0.05) at 50-70 mg/kg Mn levels. Microbial protein contents (p < 0.05) and lipase activities of Mn-Met groups were higher than those of MnSO4 and MnCl2 groups at 40-50 mg/kg Mn levels.

Discussion: Therefore, Mn-met was the best Mn source, and 40 to 50 mg/kg was the best Mn level for rumen fermentation of yaks.

Keywords: digestive enzyme activities; manganese; microbial protein; rumen fermentation in vitro; volatile fatty acids.