CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets

Weiran Su; Junru Li; Chen Ji; Congshuo Chen; Yuzheng Wang; Huili Dai; Fengqin Li; Peifeng Liu

doi:10.1007/s12274-023-5567-4

CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets

Nano Res. 2023 Mar 20:1-14. doi: 10.1007/s12274-023-5567-4. Online ahead of print.

Authors

Weiran Su^#^{1

2

3}, Junru Li^#^{1

2

3}, Chen Ji^{1

2

3}, Congshuo Chen^{1

2

3}, Yuzheng Wang^{1

2

3}, Huili Dai^{1

2

3}, Fengqin Li^{1

2

3}, Peifeng Liu^{1

2

3}

Affiliations

¹ State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200032 China.
² Central Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127 China.
³ Micro-Nano Research and Diagnosis Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127 China.

^# Contributed equally.

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are becoming powerful tools for disease biomarkers detection. Due to the specific recognition, cis-cleavage and nonspecific trans-cleavage capabilities, CRISPR/Cas systems have implemented the detection of nucleic acid targets (DNA and RNA) as well as non-nucleic acid targets (e.g., proteins, exosomes, cells, and small molecules). In this review, we first summarize the principles and characteristics of various CRISPR/Cas systems, including CRISPR/Cas9, Cas12, Cas13 and Cas14 systems. Then, various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced emphatically. Finally, the prospects and challenges of their applications in biosensing are discussed.

Keywords: clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas); detection; non-nucleic acid targets; nucleic acid targets.

Publication types

Review