Effects of graphene oxide nanoparticles administration against reserpine-induced neurobehavioral damage and oxidative stress in an animal model of Parkinson's disease

Mjid Oukhrib; Lahcen Tamegart; Abdeslam Assafi; Lamia Hejji; Abdelmonaim Azzouz; Luis Pérez Villarejo; Mohammed Haida; Chraa Mohamed; Halima Gamrani

doi:10.1016/j.fct.2023.113904

Effects of graphene oxide nanoparticles administration against reserpine-induced neurobehavioral damage and oxidative stress in an animal model of Parkinson's disease

Food Chem Toxicol. 2023 Aug:178:113904. doi: 10.1016/j.fct.2023.113904. Epub 2023 Jun 23.

Authors

Mjid Oukhrib¹, Lahcen Tamegart², Abdeslam Assafi³, Lamia Hejji⁴, Abdelmonaim Azzouz⁵, Luis Pérez Villarejo⁴, Mohammed Haida⁶, Chraa Mohamed¹, Halima Gamrani⁷

Affiliations

¹ Neurosciences, Pharmacology and Environment Team, Laboratory of Clinical, Experimental and Environmental Neurosciences, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, Morocco.
² Neurosciences, Pharmacology and Environment Team, Laboratory of Clinical, Experimental and Environmental Neurosciences, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, Morocco; Biology, Health and Ecology Laboratory, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, Tetouan, Morocco. Electronic address: l.tamegart@uae.ac.ma.
³ Department of Chemistry, Faculty of Science, University of Abdelmalek Essaadi, B.P. 2121, M'Hannech II, 93002, Tétouan, Morocco.
⁴ Department of Chemical, Environmental, and Materials Engineering, Higher Polytechnic School of Linares, University of Jaén, Campus Científico-Tecnológico, Cinturón Sur s/n, 23700, Linares (Jaén), Spain.
⁵ Department of Chemistry, Faculty of Science, University of Abdelmalek Essaadi, B.P. 2121, M'Hannech II, 93002, Tétouan, Morocco. Electronic address: aazzouz@uae.ac.ma.
⁶ Water, Biodiversity, and Climate Change Laboratory, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco.
⁷ Neurosciences, Pharmacology and Environment Team, Laboratory of Clinical, Experimental and Environmental Neurosciences, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, Morocco. Electronic address: gamrani@uca.ac.ma.

PMID: 37356558
DOI: 10.1016/j.fct.2023.113904

Abstract

Parkinson's disease (PD) is a progressive neurodegenerative disease characterized by the degeneration of substantia nigra dopaminergic neurons. Many therapeutic strategies were explored for PD with no success. In this study, we investigated the efficacy of graphene oxide nanoparticles (GONPs) using the reserpine model of PD. Low concentrations GONPs were utilized as a therapeutic agent in many neurodegenerative diseases. We assessed the neurobehavioral alterations in the reserpine model of PD and investigated the neuroprotective and antioxidant effects of GONPs in this model. Thirty male mice were separated into three groups (N = 10): C (control); Res (Reserpine 0.25 mg/kg); Res + GONPs (Reserpine 0.25 mg/kg and GONPs 25 mg/kg). Our results showed that reserpine neurotoxicity induced hypoactivity with a significant increase of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) levels in the brain and brainstem. GONPs reversed the reserpine-induced hypoactivity concomitant with decreased neuronal CAT and MDA levels. These findings support the potential use of GONPs as an antioxidant agent in the central nervous system (CNS) that protects against neurodegeneration in the reserpine PD model.

Keywords: Behavioral; Graphene oxide nanoparticles; Oxidative stress; Parkinson's disease.

MeSH terms

Animals
Antioxidants / metabolism
Disease Models, Animal
Dopaminergic Neurons
Male
Mice
Neurodegenerative Diseases*
Neuroprotective Agents* / pharmacology
Neuroprotective Agents* / therapeutic use
Oxidative Stress
Parkinson Disease* / drug therapy
Parkinson Disease* / etiology
Reserpine / therapeutic use
Reserpine / toxicity

Substances

Reserpine
graphene oxide
Antioxidants
Neuroprotective Agents