Single-cell metabolomics by mass spectrometry: ready for primetime?

Congrou Zhang; Sylvia E Le Dévédec; Ahmed Ali; Thomas Hankemeier

doi:10.1016/j.copbio.2023.102963

Single-cell metabolomics by mass spectrometry: ready for primetime?

Curr Opin Biotechnol. 2023 Aug:82:102963. doi: 10.1016/j.copbio.2023.102963. Epub 2023 Jun 23.

Authors

Congrou Zhang¹, Sylvia E Le Dévédec², Ahmed Ali³, Thomas Hankemeier⁴

Affiliations

¹ Metabolomics and Analytics Center, Leiden Academic Centre of Drug Research, Leiden University, Leiden, the Netherlands.
² Division of Drug Discovery and Safety, Leiden Academic Centre of Drug Research, Leiden University, Leiden, the Netherlands.
³ Metabolomics and Analytics Center, Leiden Academic Centre of Drug Research, Leiden University, Leiden, the Netherlands. Electronic address: ali@lacdr.leidenuniv.nl.
⁴ Metabolomics and Analytics Center, Leiden Academic Centre of Drug Research, Leiden University, Leiden, the Netherlands. Electronic address: hankemeier@lacdr.leidenuniv.nl.

PMID: 37356380
DOI: 10.1016/j.copbio.2023.102963

Abstract

Single-cell metabolomics (SCMs) is a powerful tool for studying cellular heterogeneity by providing insight into the differences between individual cells. With the development of a set of promising SCMs pipelines, this maturing technology is expected to be widely used in biomedical research. However, before SCMs is ready for primetime, there are some challenges to overcome. In this review, we summarize the trends and challenges in the development of SCMs. We also highlight the latest methodologies, applications, and sketch the perspective for integration with other omics and imaging approaches.

Publication types

Review

MeSH terms

Mass Spectrometry / methods
Metabolomics* / methods
Single-Cell Analysis* / methods