Proteomic characterization of extracellular vesicles derived from lactic acid bacteria

Bao-Hong Lee; You-Zuo Chen; Tang-Long Shen; Tzu-Ming Pan; Wei-Hsuan Hsu

doi:10.1016/j.foodchem.2023.136685

Proteomic characterization of extracellular vesicles derived from lactic acid bacteria

Food Chem. 2023 Nov 30:427:136685. doi: 10.1016/j.foodchem.2023.136685. Epub 2023 Jun 21.

Authors

Bao-Hong Lee¹, You-Zuo Chen², Tang-Long Shen³, Tzu-Ming Pan⁴, Wei-Hsuan Hsu⁵

Affiliations

¹ Department of Horticulture, National Chiayi University, Chiayi 60004, Taiwan.
² Department of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan 701401, Taiwan.
³ Department of Plant Pathology and Microbiology, National Taiwan University, Taipei 106319, Taiwan; Center for Biotechnology, National Taiwan University, Taipei 106319, Taiwan.
⁴ Department of Research and Development Division, SunWay Biotech Co., Ltd., Taipei 114067, Taiwan; Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei 106319, Taiwan.
⁵ Department of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan 701401, Taiwan; Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701401, Taiwan. Electronic address: whhsu@mail.ncku.edu.tw.

PMID: 37356267
DOI: 10.1016/j.foodchem.2023.136685

Abstract

Lactobacillus species confer health benefits by their metabolites, secreted molecules, and population numbers. Extracellular vesicles (EVs) are nano-sized particles released from cells and mediate intercellular communications. EVs-encapsulated cargos are a crucial key to decide involved biological function. However, little is known about the composition of EVs, leaving mechanisms by which Lactobacillus-derived EVs affect recipient cells remaining unresolved. This study examined the composition of EV proteins from Lactobacillus species by using liquid chromatography coupled with tandem mass spectrometry, including L. plantarum, L. fermentum, and L. gasseri. The major proteins of EVs are associated with biological processes such as catalytic activity, gluco-neogenesis, cell wall organization, and glycolytic processes. Motif enrichment analysis revealed that EVs from L. plantarum and L. fermentum contained proteins with serine-rich motif. This is the first study to report the composition and comparison of EV proteins from Lactobacillus species, providing important information of EVs in functional food products development.

Keywords: Extracellular vesicles; Lactic acid bacteria; Lactobacillus; Probiotics; Proteome profile.

MeSH terms

Chromatography, Liquid / methods
Extracellular Vesicles* / metabolism
Lactobacillales*
Lactobacillus
Proteomics / methods
Tandem Mass Spectrometry