New strategies to analyze argentatins A and B in guayule (Parthenium argentatum, A. Gray)

Beatriz Gallego; M Mercedes García-Martínez; Guayente Latorre; M Engracia Carrión; Jorge Hurtado de Mendoza; Manuel Carmona; Amaya Zalacain

doi:10.1016/j.talanta.2023.124856

New strategies to analyze argentatins A and B in guayule (Parthenium argentatum, A. Gray)

Talanta. 2023 Dec 1:265:124856. doi: 10.1016/j.talanta.2023.124856. Epub 2023 Jun 20.

Authors

Beatriz Gallego¹, M Mercedes García-Martínez², Guayente Latorre³, M Engracia Carrión⁴, Jorge Hurtado de Mendoza⁵, Manuel Carmona⁶, Amaya Zalacain⁷

Affiliations

¹ Instituto de Toxicología de La Defensa, Hospital Central de La Defensa Gómez Ulla, Gta. Ejército 1, 28047, Madrid, Spain. Electronic address: bgaligl@mde.es.
² Instituto Técnico Agronómico Provincial de Albacete, ITAP. Parque Empresarial Campollano, 2(a) Avenida, 02007, Albacete, 61, Spain; Universidad de Castilla-La Mancha, E.T.S.I. Agronómica, de Montes y Biotecnología (ETSIAMB), Cátedra de Química Agrícola, Avda. de España S/n, Albacete, 02071, Spain. Electronic address: MariaMercedes.Garcia@uclm.es.
³ Universidad de Castilla-La Mancha, E.T.S.I. Agronómica, de Montes y Biotecnología (ETSIAMB), Cátedra de Química Agrícola, Avda. de España S/n, Albacete, 02071, Spain. Electronic address: Guayente.Latorre@uclm.es.
⁴ Universidad de Castilla-La Mancha, Institute for Regional Development (IDR), Food Quality Research Group, Campus Universitario S/n, Albacete, 02071, Spain. Electronic address: MEngracia.carrion@uclm.es.
⁵ Gestión de Recursos e Innovación, Grilab, Madrid, Spain. Electronic address: jhm@grilab.es.
⁶ Universidad de Castilla-La Mancha, Institute for Regional Development (IDR), Food Quality Research Group, Campus Universitario S/n, Albacete, 02071, Spain. Electronic address: Manuel.Carmona@uclm.es.
⁷ Universidad de Castilla-La Mancha, E.T.S.I. Agronómica, de Montes y Biotecnología (ETSIAMB), Cátedra de Química Agrícola, Avda. de España S/n, Albacete, 02071, Spain. Electronic address: Amaya.Zalacain@uclm.es.

PMID: 37356192
DOI: 10.1016/j.talanta.2023.124856

Abstract

There is considerable interest in the exploitation of compounds belonging to the triterpenoid family from guayule (Parthenium argentatum, A. Gray), as they offer several beneficial effects to human health. The most abundant triterpenoids in guayule resin are the argentatins, which are currently analyzed by labor-intensive and time-consuming techniques. The purpose of the present study was to estimate argentatins and isoargentatins A and B in guayule using near-infrared spectroscopy (NIRS) and flow injection analysis (FIA). Results revealed that the best partial least squares regression model exhibited excellent correlation with the values estimated by NIRS calibration (r²c = 0.99-1.00) and cross-validation (r²cv = 0.94-0.99), and the residual predictive deviation was >3 in all cases. After optimization of the liquid chromatography-mass spectrometry and FIA parameters, the FIA mode could reliably collect data for argentatin A and B after applying a calculated coverage factor. In sum, NIRS and FIA appear to be a robust option for the estimation and routine analysis of argentatins in guayule stems and resin, respectively.

Keywords: Argentatins; Flow injection analysis; Guayule; Near infrared spectroscopy; Partial least squares regression model; Resin.

MeSH terms

Asteraceae* / chemistry
Triterpenes* / analysis

Substances

Triterpenes