Beta-agonists are potent bronchodilators approved for the treatment of asthma and tocolysis. However, they have been extensively misused as feed additives in the veterinary field to improve feed efficiency. The concern over their potential hazard to health has come to the fore again. In this study, a novel vinylene-based covalent organic framework (V-COF-1) with a two-dimensional structure was developed. The structure shows good tolerance in a variety of mediums, which can be attributed to the low polarity linkage. The high specific surface area and variable interaction with analytes accelerate the extraction time. Furthermore, the swelling resulting from the formation of hydrogen bonds by the protic solvent intercalation with the triazine group also improves the adsorption efficiency. Finally, due to its great reusability, it is economical material in sample preparation application. The V-COF-1 based μ-dSPE approach was coupled with UHPLC-MS/MS to develop a highly sensitive and selective method. The linearity of the method ranged from 0.05 to 20 ng g-1 with a correlation coefficient (R2) higher than 0.9958, and the limits of detection and quantification fell in the ranges of 0.01-0.10 ng g-1 and 0.04-0.32 ng g-1. The proposed method has been successfully applied to determine beta-agonists in meat samples, and the results indicated good recovery of 82.2-116%. The intra-day and inter-day precision were less than 6.61%, indicating the potential for sustainable application in food analysis.
Keywords: Beta-agonists; Micro dispersive solid-phase extraction; UHPLC–MS/MS; Vinylene-based covalent organic framework.
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