A magnetic solid-phase extraction (MSPE) method was developed for the enrichment of trace amounts of estrone (E1), 17-β-estradiol (E2) and bisphenol A (BPA) from aqueous samples using the Rubber-Fe3O4@SiO2@Mg-Al Layered double hydroxide followed by determination by HPLC. The proposed sorbent was characterized by X-ray diffraction, Field emission scanning electron microscopy, Fourier transform infrared spectroscopy, Vibrating-sample magnetometer, Thermal gravimetric analysis, N2 adsorption/desorption Brunauer-Emmett-Teller surface analysis, Barrett-Joyner-Halenda pore size analysis and Energy dispersive X-ray spectroscopy. Factors affecting the extraction efficiency such as pH, ionic strength, extraction temperature and time, desorption solvent and time were optimized. The limit of detection and quantification were obtained as 0.33, 1 µg L-1 for the E1 and E2 and BPA, respectively. Also, linear range of the method were 1-150, 1-100 and 1-150 µg L-1for E1, E2, and BPA, respectively. Relative standard deviations (RSD%) for the repeatability of extraction on one sorbent were obtained as 2.98, 1.31 and 3.50%, also, sorbent to sorbent repeatability were investigated and RSD% values were obtained as 7.58, 8.35 and 8.12% for E1, E2 and BPA, respectively.
Keywords: 17-β-estradiol; Bisphenol A; Estrone; Layered double hydroxide; Magnetic solid-phase extraction.
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