MXenes-Au NPs modified electrochemical biosensor for multiple exosome surface proteins analysis

Zhangmin Wang; Ze Zhang; Yingcong Zhang; Xin Xu; Tong Shen; Hongzhi Pan; Dong Chang

doi:10.1016/j.talanta.2023.124848

MXenes-Au NPs modified electrochemical biosensor for multiple exosome surface proteins analysis

Talanta. 2023 Dec 1:265:124848. doi: 10.1016/j.talanta.2023.124848. Epub 2023 Jun 20.

Authors

Zhangmin Wang¹, Ze Zhang¹, Yingcong Zhang², Xin Xu¹, Tong Shen¹, Hongzhi Pan³, Dong Chang⁴

Affiliations

¹ Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, China.
² Department of Clinical Laboratory, Huashan Hospital, Fudan University, Shanghai, 200040, China.
³ Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201399, China. Electronic address: panhongzhi@163.com.
⁴ Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, 201399, China. Electronic address: changdongydy@163.com.

PMID: 37352782
DOI: 10.1016/j.talanta.2023.124848

Abstract

As a novel class of non-invasive biomarkers, exosome-carried proteins are essential in early detection and precise cancer diagnosis. In the study, we developed an electrochemical biosensor based on MXenes-Au NPs modification to assess the differential expression of EGFR, CEA, and EpCAM proteins of exosomes. This sensor has sensitively detected tumor biomarkers in the exosomes generated by various tumor cells (including A549, MCF-7, PC-3, and HeLa). Building a biosensor that can distinguish minute differences of proteins in various derived-from exosomes is crucial for addressing the issues with early and accurate cancer detection.

Keywords: Electrochemical biosensor; Exosomes; MXenes-Au NPs.

MeSH terms

Biomarkers, Tumor / analysis
Biosensing Techniques*
Exosomes* / chemistry
HeLa Cells
Humans
Membrane Proteins / metabolism

Substances

Membrane Proteins
Biomarkers, Tumor