Ishophloroglucin A-based multifunctional oxidized alginate/gelatin hydrogel for accelerating wound healing

Nam-Gyun Kim; Se-Chang Kim; Tae-Hee Kim; Jae-Young Je; Bonggi Lee; Sang Gil Lee; Young-Mog Kim; Hyun Wook Kang; Zhong-Ji Qian; Namwon Kim; Won-Kyo Jung

doi:10.1016/j.ijbiomac.2023.125484

Ishophloroglucin A-based multifunctional oxidized alginate/gelatin hydrogel for accelerating wound healing

Int J Biol Macromol. 2023 Aug 1:245:125484. doi: 10.1016/j.ijbiomac.2023.125484. Epub 2023 Jun 20.

Authors

Nam-Gyun Kim¹, Se-Chang Kim¹, Tae-Hee Kim², Jae-Young Je³, Bonggi Lee⁴, Sang Gil Lee⁵, Young-Mog Kim⁶, Hyun Wook Kang⁷, Zhong-Ji Qian⁸, Namwon Kim⁹, Won-Kyo Jung¹⁰

Affiliations

¹ Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence and New-Senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea.
² Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea.
³ Major of Human Bioconvergence, School of Smart Healthcare, Pukyong National University, Busan 48513, South Korea.
⁴ Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea.
⁵ Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea; Department of Smart Green Technology Engineering, Pukyong National University, Busan, 48513, South Korea.
⁶ Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea; Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea.
⁷ Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence and New-Senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea.
⁸ College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Ocean University, Zhanjiang 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518114, Guangdong, China.
⁹ Ingram School of Engineering, Texas State University, San Marcos, TX 78666, USA; Research Institute of Advanced Materials (RIAM), Seoul National University, Seoul 08826, Republic of Korea; Materials Science, Engineering, and Commercialization (MSEC), Texas State University, San Marcos, TX 78666, USA.
¹⁰ Major of Biomedical Engineering, Division of Smart Healthcare, College of Information Technology and Convergence and New-Senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, Republic of Korea; Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, Republic of Korea. Electronic address: wkjung@pknu.ac.kr.

PMID: 37348579
DOI: 10.1016/j.ijbiomac.2023.125484

Abstract

This study investigated the potential applicability of wound dressing hydrogels for tissue engineering, focusing on their ability to deliver pharmacological agents and absorb exudates. Specifically, we explored the use of polyphenols, as they have shown promise as bioactive and cross-linking agents in hydrogel fabrication. Ishophloroglucin A (IPA), a polyphenol not previously utilized in tissue engineering, was incorporated as both a drug and cross-linking agent within the hydrogel. We integrated the extracted IPA, obtained through the utilization of separation and purification techniques such as high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR) into oxidized alginate (OA) and gelatin (GEL) hydrogels. Our findings revealed that the mechanical properties, thermal stability, swelling, and degradation of the multifunctional hydrogel can be modulated via intermolecular interactions between the natural polymer and IPA. Moreover, the controlled release of IPA endows the hydrogel with antioxidant and antimicrobial characteristics. Overall, the wound healing efficacy, based on intermolecular interactions and drug potency, has been substantiated through accelerated wound closure and collagen deposition in an ICR mouse full-thickness wound model. These results suggest that incorporating IPA into natural polymers as both a drug and cross-linking agent has significant implications for tissue engineering applications.

Keywords: Gelatin; Ishophloroglucin A (IPA); Non-covalent interaction; Oxidized alginate; Wound healing.

MeSH terms

Alginates / chemistry
Animals
Anti-Bacterial Agents
Gelatin* / chemistry
Hydrogels* / chemistry
Mice
Mice, Inbred ICR
Wound Healing

Substances

Hydrogels
Gelatin
Alginates
ishophloroglucin A
Anti-Bacterial Agents