Microfluidic based single cell or droplet manipulation: Methods and applications

Yuwei Lan; Yang Zhou; Man Wu; Chunping Jia; Jianlong Zhao

doi:10.1016/j.talanta.2023.124776

Microfluidic based single cell or droplet manipulation: Methods and applications

Talanta. 2023 Dec 1:265:124776. doi: 10.1016/j.talanta.2023.124776. Epub 2023 Jun 15.

Authors

Yuwei Lan¹, Yang Zhou², Man Wu³, Chunping Jia⁴, Jianlong Zhao⁵

Affiliations

¹ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: lanyuwei@mail.sim.ac.cn.
² State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: yzhou@mail.sim.ac.cn.
³ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China. Electronic address: wuman@mail.sim.ac.cn.
⁴ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: jiachp@mail.sim.ac.cn.
⁵ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, 100101, China. Electronic address: jlzhao@mail.sim.ac.cn.

PMID: 37348357
DOI: 10.1016/j.talanta.2023.124776

Abstract

The isolation of single cell or droplet is first and crucial step to single-cell analysis, which is important for cancer research and diagnostic methods. This review provides an overview of technologies that are currently used or in development to realize the isolation. Microfluidic based manipulation is an emerging technology with the distinct advantages of miniaturization and low cost. Therefore, recent developments in microfluidic isolated methods have attracted extensive attention. We introduced herein five strategies based on microfluid: trap, microfluidic discrete manipulation, bioprinter, capillary and inertial force. For every technology, their basic principles and features were discussed firstly. Then some modified approaches and applications were listed as the extension. Finally, we compared the advantages and drawbacks of these methods, and analyzed the trend of the manipulation based on microfluidics.

Keywords: Cell isolation; Microfluidic control; Particle manipulation; Single-cell.

Publication types

Review

MeSH terms

Microfluidic Analytical Techniques*
Microfluidics* / methods
Miniaturization
Single-Cell Analysis