In this study, polylactic acid (PLA) microspheres were used as the raw material to construct bulk implants with surface microtopography through hot pressing and heat treatment, and the microtopographical structures were regulated through the sizes of the PLA microspheres. The surface microtopographies of PLA implants were successfully constructed using micron-sized bulges, which showed a wave-like structure. The ridge width of bulges ranged from 1.64 ± 0.16 µm to 82.52 ± 14.38 µm and the valley depth ranged from 0.49 ± 0.07 µm to 37.35 ± 6.78 µm according to the sizes of microspheres. The nanoindentation tests showed that the modulus and hardness of PLA implants were gradually increased with the decrease in microsphere sizes. The surface microtopography resulted in a slight increase in the hydrophobicity of the PLA implants, but no significant differences were observed. Cells cultured on the implant surface with microtopography exhibited varying morphological responses, and significantly increased osteogenic activity was observed relative to a PLA flat film. This study demonstrated that the surface microtopography derived from PLA microspheres could regulate cellular response and activate osteogenic properties of PLA implants.
Keywords: Morphological responses; Mouse embryonic osteoblast cell; Osteogenic differentiation; Surface microtopographies.
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