A synthetic Tn-BSA conjugate vaccine bearing chitotriose as built-in adjuvant

Song Bai; Hang Gao; Lin-Tong-Qing Tan; Lulu Yao; Xin Meng; Yongzhong Zhang

doi:10.1016/j.carres.2023.108875

A synthetic Tn-BSA conjugate vaccine bearing chitotriose as built-in adjuvant

Carbohydr Res. 2023 Aug:530:108875. doi: 10.1016/j.carres.2023.108875. Epub 2023 Jun 16.

Authors

Song Bai¹, Hang Gao², Lin-Tong-Qing Tan², Lulu Yao¹, Xin Meng³, Yongzhong Zhang⁴

Affiliations

¹ Institute of Disaster and Emergency Medicine, Tianjin University, Tianjin, 300072, PR China.
² Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Tianjin, 300457, PR China.
³ Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, Tianjin, 300457, PR China. Electronic address: mengxin@tust.edu.cn.
⁴ Institute of Disaster and Emergency Medicine, Tianjin University, Tianjin, 300072, PR China. Electronic address: zyzzjx@163.com.

PMID: 37348181
DOI: 10.1016/j.carres.2023.108875

Abstract

Chitotriose (CTS), the hydrolysate of chitosan, is readily soluble in water because of the shorter chain lengths of the oligomers and the free amino groups in the d-glucosamine units. In the current study, we report the synthesis of novel conjugate vaccine Tn-BSA-CTS with chitotriose as built-in adjuvant, along with an evaluation of the effect of adjuvant chitotriose (CTS). Immunological evaluations of the resultant conjugate vaccine revealed that Tn-BSA-CTS could provoke the highest titers of IgG antibodies (102,400). The Tn-BSA-CTS conjugate remarkably enhanced both humoral and cellular immunity. The obtained results demonstrate the potential of CTS as a novel vaccine adjuvant in the development of antitumor vaccine and the covalent linkage of tumor vaccine to CTS might be available strategy to increase the efficacy against cancer.

Keywords: Adjuvant; Chitotriose; Conjugate vaccine; Glycoconjugate; Tn antigen.

MeSH terms

Adjuvants, Immunologic* / pharmacology
Cancer Vaccines*
Trisaccharides
Vaccines, Conjugate

Substances

Vaccines, Conjugate
chitotriose
Adjuvants, Immunologic
Trisaccharides
Cancer Vaccines