EBR and JA regulate aroma substance biosynthesis in 'Ruidu Hongyu' grapevine berries by transcriptome and metabolite combined analysis

Jiajia Li; Yi Quan; Zishu Wu; Jiayu Han; Ying Zhang; Hafiz Umer Javed; Chao Ma; Songtao Jiu; Caixi Zhang; Lei Wang; Shiping Wang

doi:10.3389/fpls.2023.1185049

EBR and JA regulate aroma substance biosynthesis in 'Ruidu Hongyu' grapevine berries by transcriptome and metabolite combined analysis

Front Plant Sci. 2023 Jun 6:14:1185049. doi: 10.3389/fpls.2023.1185049. eCollection 2023.

Authors

Jiajia Li¹, Yi Quan¹, Zishu Wu¹, Jiayu Han², Ying Zhang², Hafiz Umer Javed³, Chao Ma¹, Songtao Jiu¹, Caixi Zhang¹, Lei Wang¹, Shiping Wang¹

Affiliations

¹ Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
² Grape and Wine Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China.
³ College of Chemistry and Chemical Engineering, Zhongkai University of Agricultural Engineering, Guangzhou, China.

Abstract

Volatile compounds including terpenes, aldehyde, phenol, and alcohol are significantly contributed floral and fruity aromas to the Muscat variety. 'Ruidu Hongyu' grapevine is one of the newly developed grape varieties, and cultivation of this variety has been extended across China due to unique quality traits and taste. In this study, HS-SPME/GC-MS and transcriptome sequencing analysis were performed to evaluate the impact of exogenous 2,4-epibrassinolide (EBR), jasmonic acid (JA), and their signaling inhibitors brassinazole (Brz)/sodium diethyldithiocarbamate (DIECA) on the biosynthesis of aroma substances in 'Ruidu Hongyu' grapevine. According to the results, exogenous BR and JA promoted the accumulation of various aroma substances, including hexenal, 2-hexenal, nerol oxide, vanillin, hotrienol, terpineol, neral, nerol, geraniol, and geranic acid. After EBR and JA treatments, most of the genes responsible for terpene, aldehyde, and alcohol biosynthesis expressed at a higher level than the CK group. Relatively, EBR treatment could not only promote endogenous BR biosynthesis and metabolism but also elevate BR signaling transduction. JA treatment contributed to endogenous JA and MeJA accumulation, as well. Through transcriptome sequencing, a total of 3043, 903, 1470, and 607 DEGs were identified in JA vs. JD, JA vs. CK, BR vs. CK, and BR vs. Brz, respectively. There were more DEGs under both EBR and JA treatments at late fruit ripening stages. The findings of this study increase our understanding regarding aroma substances biosynthesis and endogenous BR/JA metabolism in response to exogenous EBR and JA signals.

Keywords: EBR; HS-SPME/GC−MS; JA; aroma; grapevine; transcriptome sequencing; ‘Ruidu Hongyu’.

Grants and funding

This study was supported by the Action Plan for Science and Technology Innovation of Shanghai Science and Technology Commission (Grant No. 22015820400); Ningxia Hui Autonomous Region Key Project of Research and Development Plan (Grant No.2022BBF02013); The Work Station for characteristic grape industries in Wenping Xu plateau, Yunnan (Grant No. YSZJGZZ-2021061); The China Agriculture Research System of MOF and MARA (CARS-29-ZP-7).