Influence of Carboxyl Group Ratios on the Design of Breast Cancer Targeting Bimodal MR/NIR-II Imaging Probe from PLGA@Gd-DOTA@PEG Micelles Conjugating Herceptin

ACS Appl Bio Mater. 2023 Jul 17;6(7):2644-2650. doi: 10.1021/acsabm.3c00260. Epub 2023 Jun 22.

Abstract

We developed a small MRI/NIR-II probe to target HER2 (tetanucleotide) breast cancer cells. The probe is composed of PLGA-b-PEG micelles encapsulated NIR-II, and Gd-DOTA is conjugated at the border of PLGA/PEG. Herceptin was then conjugated to carboxyl residues of PLGA-b-PEG chains. We examined the influence of carboxyl group ratios on the probe property stability and Herceptin concentration and the binding affinity to HER2(+) cells corresponding to the -COOH ratios. The binding assays demonstrated that the optimal surface ratio of -COOH is 5%, which is less affected by fluorescence reduction and which exhibited the highest antigen-capturing activity.

Keywords: Herceptin; MRI; NIR-II; bioimaging; breast cancer.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms* / diagnostic imaging
  • Breast Neoplasms* / drug therapy
  • Female
  • Humans
  • Magnetic Resonance Imaging
  • Micelles
  • Trastuzumab / chemistry
  • Trastuzumab / pharmacology
  • Trastuzumab / therapeutic use

Substances

  • Trastuzumab
  • poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer
  • Micelles
  • gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate