Deciphering anti-infectious compounds from Peruvian medicinal Cordoncillos extract library through multiplexed assays and chemical profiling

Pedro G Vásquez-Ocmín; Sandrine Cojean; Vincent Roumy; Guillaume Marti; Sébastien Pomel; Alice Gadea; Karine Leblanc; Indira Dennemont; Liliana Ruiz-Vásquez; Hivelli Ricopa Cotrina; Wilfredo Ruiz Mesia; Stéphane Bertani; Lastenia Ruiz Mesia; Alexandre Maciuk

doi:10.3389/fphar.2023.1100542

Deciphering anti-infectious compounds from Peruvian medicinal Cordoncillos extract library through multiplexed assays and chemical profiling

Front Pharmacol. 2023 Jun 5:14:1100542. doi: 10.3389/fphar.2023.1100542. eCollection 2023.

Authors

Pedro G Vásquez-Ocmín¹, Sandrine Cojean^{2

3}, Vincent Roumy⁴, Guillaume Marti^{5

6}, Sébastien Pomel², Alice Gadea¹, Karine Leblanc², Indira Dennemont², Liliana Ruiz-Vásquez^{7

8}, Hivelli Ricopa Cotrina⁸, Wilfredo Ruiz Mesia⁹, Stéphane Bertani^{1

10}, Lastenia Ruiz Mesia^{8

9}, Alexandre Maciuk²

Affiliations

¹ UMR152 PHARMADEV, IRD, UPS, Université de Toulouse, Toulouse, France.
² Université Paris-Saclay, CNRS, BioCIS, Orsay, France.
³ CNR Du Paludisme, AP-HP, Hôpital Bichat-Claude Bernard, Paris, France.
⁴ Joint Research Unit 1158 BioEcoAgro, University Lille, JUNIA, INRAE, University Liège, UPJV, University Artois, ULCO, VilleneuveD'Ascq, France.
⁵ Laboratoire de Recherche en Sciences Végétales (UMR 5546), CNRS, Université de Toulouse, Toulouse, France.
⁶ MetaboHUB, National Infrastructure of Metabolomics and Fluxomics, Toulouse, France.
⁷ Facultad de Farmacia y Bioquímica, Universidad Nacional de la Amazonía Peruana (UNAP), Iquitos, Peru.
⁸ Centro de Investigación de Recursos Naturales, Universidad Nacional de la Amazonía Peruana (UNAP), Iquitos, Peru.
⁹ Facultad de Ingeniería Química, Universidad Nacional de la Amazonía Peruana (UNAP), Iquitos, Peru.
¹⁰ International Joint Laboratory of Molecular Anthropological Oncology (LOAM), National Cancer Institute, Lima, Perú.

Abstract

High prevalence of parasitic or bacterial infectious diseases in some world areas is due to multiple reasons, including a lack of an appropriate health policy, challenging logistics and poverty. The support to research and development of new medicines to fight infectious diseases is one of the sustainable development goals promoted by World Health Organization (WHO). In this sense, the traditional medicinal knowledge substantiated by ethnopharmacology is a valuable starting point for drug discovery. This work aims at the scientific validation of the traditional use of Piper species ("Cordoncillos") as firsthand anti-infectious medicines. For this purpose, we adapted a computational statistical model to correlate the LCMS chemical profiles of 54 extracts from 19 Piper species to their corresponding anti-infectious assay results based on 37 microbial or parasites strains. We mainly identified two groups of bioactive compounds (called features as they are considered at the analytical level and are not formally isolated). Group 1 is composed of 11 features being highly correlated to an inhibiting activity on 21 bacteria (principally Gram-positive strains), one fungus (C. albicans), and one parasite (Trypanosoma brucei gambiense). The group 2 is composed of 9 features having a clear selectivity on Leishmania (all strains, both axenic and intramacrophagic). Bioactive features in group 1 were identified principally in the extracts of Piper strigosum and P. xanthostachyum. In group 2, bioactive features were distributed in the extracts of 14 Piper species. This multiplexed approach provided a broad picture of the metabolome as well as a map of compounds putatively associated to bioactivity. To our knowledge, the implementation of this type of metabolomics tools aimed at identifying bioactive compounds has not been used so far.

Keywords: Cordoncillos; Peruvian amazonia; Piper; anti-infectious diseases; metabolomics.

Grants and funding

Authors acknowledge the funding support of the Universidad Nacional de la Amazonia Peruana (UNAP) (Resolución Rectoral N°1312-2020-UNAP, 9/12/2020) and Financial support from the French National Infrastructure for Metabolomics and Fluxomics, Grant MetaboHUB-ANR-11-INBS-0010.