Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method

Fan Meng; Jiao Guo; Shan Wang; Changhui Zhang; Xuehui Li; Yanjuan Chen; Qiuli Shi; Liang Shao; Rundong Zhang; Ganggang Wang; Dan Su

doi:10.1016/j.heliyon.2023.e17364

Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method

Heliyon. 2023 Jun;9(6):e17364. doi: 10.1016/j.heliyon.2023.e17364. Epub 2023 Jun 16.

Authors

Fan Meng¹, Jiao Guo¹, Shan Wang¹, Changhui Zhang², Xuehui Li², Yanjuan Chen², Qiuli Shi², Liang Shao², Rundong Zhang², Ganggang Wang³, Dan Su^{2

4}

Affiliations

¹ Jujing-Chengdu Biotech, Chengdu, Sichuan, PR China.
² State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, PR China.
³ Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, PR China.
⁴ Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, PR China.

Abstract

The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has had a significant impact on global health. To address the urgent need for plasmids containing SARS-CoV-2 sequences in research, we have developed a high-throughput FastCloning platform for the construction of associated plasmids. Our platform uses a FastCloning method to construct a plasmid library from 29 ORFs of the virus and 20 commonly used vectors in the lab. The library contains 536 recombinant vectors, with a highly positive clone success rate of 92.4%. Our study provides a rapid and efficient approach to constructing a large plasmid library for SARS-CoV-2 research.

Keywords: COVID-19; Parallel cloning; Plasmids library; SARS-CoV-2.