Microbial biofilm development on the membrane surface of bioreactors results in membrane flux decline (biofouling). Biofouling is one of the most severe problems limiting the use of these bioreactors. For detailed understanding of the biofouling, microbial community and dissolved organic matter analyses have been performed in recent decades. Although most previous studies have focused on mature biofilms at the end point of biofouling, understanding of the early stages is crucial to mitigate biofilm formation. Thus, recent studies have focused on the impact of early-stage biofilm development and indicated a clear difference in microbial communities between early-stage and mature biofilms. In addition, certain bacteria play a significant role in early-stage biofilms. The present mini-review systematically summarizes the foulants present during early-stage fouling, provides novel perspectives on fouling mechanisms, and discusses the neglected effect of planktonic bacteria.
Keywords: Early-stage biofilm; pioneer bacteria; soluble microbial products; transparent exopolymer particle.