Here, we introduce UF_RH5, a novel lytic phage targeting clinically isolated Pseudomonas aeruginosa. It belongs to the Siphovirus morphology family, Septimatrevirus genus, with a 42,566-bp genome with a GC content of 53.60%, encoding 58 proteins. Under electron microscopy, UF_RH5 exhibits a length of 121 nm and a capsid size of 45 nm.