Antibiotic resistome associated with influencing factors in industrial-scale organic waste aerobic composting plants

Jun Yang; Jinyi Xiang; Karina Yew-Hoong Gin; Yu Xie; Kaifeng Yu; Peng Li; Yiliang He

doi:10.1016/j.biortech.2023.129354

Antibiotic resistome associated with influencing factors in industrial-scale organic waste aerobic composting plants

Bioresour Technol. 2023 Oct:385:129354. doi: 10.1016/j.biortech.2023.129354. Epub 2023 Jun 17.

Authors

Jun Yang¹, Jinyi Xiang², Karina Yew-Hoong Gin³, Yu Xie⁴, Kaifeng Yu⁴, Peng Li⁴, Yiliang He⁵

Affiliations

¹ School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Department of Civil and Environmental Engineering, National University of Singapore, 1 Engineering Drive 2, Singapore 117576, Singapore.
² School of Medicine, Shanghai Jiao Tong University, 227 South Chongqing Road, Shanghai 200025, China.
³ Department of Civil and Environmental Engineering, National University of Singapore, 1 Engineering Drive 2, Singapore 117576, Singapore.
⁴ School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
⁵ School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; China-UK Low Carbon College, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China. Electronic address: ylhe@sjtu.edu.cn.

PMID: 37336453
DOI: 10.1016/j.biortech.2023.129354

Abstract

This study investigated the fate of antibiotic resistance genes (ARGs) and bacterial evolution in six industrial-scale organic wastes aerobic composting plants and identified key factors driving ARGs dynamics. A total of 226 ARGs and 46 mobile genetic elements (MGEs), mainly resistant to aminoglycoside and MLSB, were detected by high-throughput qPCR. Briefly, aerobic composting showed good performance in reducing the diversity and abundance of ARGs, where the total absolute abundance was reduced by 88.34%-97.08% except for cattle manures. Rapid composting may lead to a rebound of ARGs due to long-term storage compared to traditional composting. Hub ARGs and bacterial genera were screened out by co-occurrence patterns. As the dominant phyla in composting, the main potential hosts of ARGs were Firmicutes, Bacteroidota and Proteobacteria. Structural equation model indicated that MGEs and heavy metals were key factors affecting ARGs dynamics. In addition, nutrients and bacterial α-diversity can indirectly influence ARGs by affecting MGEs.

Keywords: Aerobic composting; Antibiotic resistance genes; Food waste; Industrial-scale; Manure.

MeSH terms

Animals
Anti-Bacterial Agents / analysis
Anti-Bacterial Agents / pharmacology
Bacteria
Cattle
Composting*
Genes, Bacterial* / genetics
Industrial Waste / analysis
Manure / microbiology

Substances

Anti-Bacterial Agents
Industrial Waste
Manure