Genetic diversity analysis of lychnis mottle virus and first identification of Angelica sinensis infection

Weijie Jin; Yubao Zhang; Xuesi Su; Zhongkui Xie; Ruoyu Wang; Zhongpei Du; Yajun Wang; Yang Qiu

doi:10.1016/j.heliyon.2023.e17006

Genetic diversity analysis of lychnis mottle virus and first identification of Angelica sinensis infection

Heliyon. 2023 Jun 5;9(6):e17006. doi: 10.1016/j.heliyon.2023.e17006. eCollection 2023 Jun.

Authors

Weijie Jin^{1

2

3}, Yubao Zhang^{1

3}, Xuesi Su^{1

2

3}, Zhongkui Xie^{1

3}, Ruoyu Wang^{1

3}, Zhongpei Du⁴, Yajun Wang^{1

3}, Yang Qiu^{1

3}

Affiliations

¹ Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Gansu Gaolan Field Scientific Observation and Research Station for Agricultural Ecosystem, Lanzhou, 730000, China.
⁴ Jiuquan Vocational and Technical College, Jiuquan, 735000, China.

Abstract

Gansu Province is a district renowned for the cultivation of Angelica sinensis (Oliv.) Diels, accounting for greater than 90% of China's total annual production. However, virus infection has caused a reduction in A. sinensis yield. Here, we collected suspected virus-infected A. sinensis leaf samples from Gansu Province's A. sinensis cultivation area. For the first time, using small RNA deep sequencing and RT-PCR, lychnis mottle virus (LycMoV) was found to naturally infect A. sinensis. The coat protein (cp) gene of the Gansu A. sinensis LycMoV isolate was obtained through cloning, where its nucleotide and amino acid identity was highest while having the closest affinity to the China Pearl (i.e., Prunus persica) isolate. Recombination analysis indicated that genetic recombination had only a limited influencing effect on the molecular evolution of LycMoV. Moreover, results from genetic diversity analysis indicated that the host, geographic isolation, and genetic drift may be the main factors that contributed to the formation of genetic diversity and differentiation in LycMoV. Furthermore, the LycMoV population trend was expansionary. Selection pressure may also be the main driver for the evolution of the entire LycMoV population, while the driving effect of genetic recombination is limited. This study marks a new LycMoV host (i.e., A. sinensis) for the first time and provides scientific support for the identification, prevention, and control of LycMoV.

Keywords: Angelica sinensis (Oliv.) Diels; Coat protein; Genetic diversity; Lychnis mottle virus; Virus population.