C-doped ZnO nanocomposites molecularly imprinted photoelectrochemical sensor for ultrasensitive and selective detection of oxytetracycline in milk

Huafang Zhang; Qinghong Pan; Wanying Cai; Xiaowei Shi; Da-Peng Yang; Hetong Lin; Enhui Qiu

doi:10.1016/j.foodchem.2023.136535

C-doped ZnO nanocomposites molecularly imprinted photoelectrochemical sensor for ultrasensitive and selective detection of oxytetracycline in milk

Food Chem. 2023 Nov 15:426:136535. doi: 10.1016/j.foodchem.2023.136535. Epub 2023 Jun 9.

Authors

Huafang Zhang¹, Qinghong Pan², Wanying Cai³, Xiaowei Shi³, Da-Peng Yang⁴, Hetong Lin⁵, Enhui Qiu⁶

Affiliations

¹ Key Laboratory of Chemical Materials and Green Nanotechnology, College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
² Key Laboratory of Chemical Materials and Green Nanotechnology, College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China.
³ The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China.
⁴ Key Laboratory of Chemical Materials and Green Nanotechnology, College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China; School of Rehabilitation Science and Engineering, University of Health and Rehabilitation Sciences, Qingdao, Shandong 266024, China. Electronic address: yangdp@qztc.edu.cn.
⁵ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: hetonglin@163.com.
⁶ The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China. Electronic address: qiuenhui110@126.com.

PMID: 37331139
DOI: 10.1016/j.foodchem.2023.136535

Abstract

Antibiotic monitoring remains vital to ensure human health and safety in the environment and foods. As the most popular detection method, photoelectrochemical (PEC) sensor can achieve rapid and accurate detection of antibiotics with the advantages of high sensitivity, easy-to-preparation process, as well as high selectivity. Herein, an extremely-efficient visible-light responsible ZnO/C nanocomposite was prepared and combined with acetylene black (as an enhanced conductive matrix), and the electron migration efficiency was greatly accelerated. Meanwhile, a molecularly imprinted polymer obtained by electrical agglomeration was conjugated as a specific recognizing site for target. Furthermore, the as-prepared rMIP-PEC sensor showed a low detection limit (8.75 pmol L^-1, S/N = 3) in a wide linear detection range of 0.01-1000 nmol L^-1 for oxytetracycline (OTC), with excellent selectivity and long-term stability. Our work shed light on applying C-doped ZnO semiconductor and molecularly imprinted polymer as photoelectric active sensing materials for rapid and accurate analysis of antibiotics in foods and environment.

Keywords: Acetylene black; Oxytetracycline; RMIP-PEC sensor; ZnO/C.

MeSH terms

Animals
Anti-Bacterial Agents / analysis
Biosensing Techniques* / methods
Electrochemical Techniques / methods
Humans
Limit of Detection
Milk / chemistry
Molecular Imprinting* / methods
Molecularly Imprinted Polymers
Nanocomposites*
Oxytetracycline* / analysis
Zinc Oxide*

Substances

Oxytetracycline
Zinc Oxide
Molecularly Imprinted Polymers
Anti-Bacterial Agents