The preparation of Diacylglycerol-rich soybean oil by acetylated modification of arachin nanoparticles for W/O Pickering emulsion system

Food Chem. 2023 Nov 15:426:136615. doi: 10.1016/j.foodchem.2023.136615. Epub 2023 Jun 12.

Abstract

Pickering emulsion catalytic system (PEC) stabilized by nanoparticles is an efficient catalytic platform. Herein, a high-performance PEC was constructed by acetylated modification of arachin nanoparticles (AAPs). The results showed the pI of arachin was decreased from pH 5.5 to pH 3.5. The surface hydrophobicity index was significantly increased (from 56.28 ± 4.23 to 120.77 ± 0.79) after acetylated modification. The three-phase contact angle of AAPs was 91.20 ± 0.98°. AAPs were used as lipase immobilization carriers to increase the activity of free lipase fabricating lipase-AAPs. The immobilization efficiency and activity of lipase-AAPs were 12.95 ± 0.03% and 1.74 ± 0.07 U/mg, respectively. Enzymatic reaction kinetics showed that Vm of lipase-AAPs was twice of free lipase. Km was 1/5 of free lipase. The catalytic efficiency of PEC to prepare DAG was 2.36 times of biphasic catalytic system (BCS). This work provided a promising way to promote the efficiency of DAG preparation.

Keywords: Acetylated modification of arachin nanoparticles; Diacylglycerol; Hydrolysis; Lipase immobilization; W/O Pickering emulsion.

MeSH terms

  • Diglycerides
  • Emulsions
  • Lipase
  • Nanoparticles*
  • Soybean Oil*

Substances

  • Soybean Oil
  • Arachin
  • Emulsions
  • Diglycerides
  • Lipase