Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances

Xiaoyu Wei; Zhen Chen; Ailing Liu; Lijie Yang; Yiyuan Xu; Mingfeng Cao; Ning He

doi:10.1016/j.biotechadv.2023.108199

Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances

Biotechnol Adv. 2023 Oct:67:108199. doi: 10.1016/j.biotechadv.2023.108199. Epub 2023 Jun 15.

Authors

Xiaoyu Wei¹, Zhen Chen², Ailing Liu¹, Lijie Yang¹, Yiyuan Xu¹, Mingfeng Cao³, Ning He⁴

Affiliations

¹ Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China.
² College of Life Science, Xinyang Normal University, Xinyang 464000, China. Electronic address: chenzhen@xynu.edu.cn.
³ Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China; Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen 361005, China. Electronic address: mfcao@xmu.edu.cn.
⁴ Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China. Electronic address: hening@xmu.edu.cn.

PMID: 37330153
DOI: 10.1016/j.biotechadv.2023.108199

Abstract

Extracellular polymeric substances are mainly synthesized via a variety of biosynthetic pathways in bacteria. Bacilli-sourced extracellular polymeric substances, such as exopolysaccharides (EPS) and poly-γ-glutamic acid (γ-PGA), can serve as active ingredients and hydrogels, and have other important industrial applications. However, the functional diversity and widespread applications of these extracellular polymeric substances, are hampered by their low yields and high costs. Biosynthesis of extracellular polymeric substances is very complex in Bacillus, and there is no detailed elucidation of the reactions and regulations among various metabolic pathways. Therefore, a better understanding of the metabolic mechanisms is required to broaden the functions and increase the yield of extracellular polymeric substances. This review systematically summarizes the biosynthesis and metabolic mechanisms of extracellular polymeric substances in Bacillus, providing an in-depth understanding of the relationships between EPS and γ-PGA synthesis. This review provides a better clarification of Bacillus metabolic mechanisms during extracellular polymeric substance secretion and thus benefits their application and commercialization.

Keywords: Extracellular polymeric substances; biosynthesis; exopolysaccharides; metabolic engineering; poly-γ-glutamic acid.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Bacillus* / genetics
Bacillus* / metabolism
Bacteria / metabolism
Extracellular Polymeric Substance Matrix / metabolism
Metabolic Engineering
Metabolic Networks and Pathways
Polyglutamic Acid / genetics
Polyglutamic Acid / metabolism

Substances

Polyglutamic Acid