Proteome reveals the mechanism of selenium-sulfur interaction in regulating isothiocyanate biosynthesis and the physiological metabolism of broccoli sprouts

Abstract

Broccoli sprouts have a strong ability to accumulate isothiocyanate and selenium. In this study, the isothiocyanate content increased significantly as a result of ZnSO₄ stress. Particularly, based on the isothiocyanate content is not affected, the combined ZnSO₄ and Na₂SeO₃ treatment alleviated the inhibition of ZnSO₄ and induced selenium content. Gene transcription and protein expression analyses revealed the changes in isothiocyanate and selenium metabolite levels in broccoli sprouts. ZnSO₄ combined with Na₂SeO₃ was proven to activate a series of isothiocyanate metabolite genes (UGT74B1, OX1, and ST5b) and selenium metabolite genes (BoSultr1;1, BoCOQ5-2, and BoHMT1). The relative abundance of the total 317 and 203 proteins, respectively, in 4-day-old broccoli sprouts varied, and the metabolic and biosynthetic pathways for secondary metabolites were significantly enriched in ZnSO₄/control and ZnSO₄ combined Na₂SeO₃/ZnSO₄ comparisons. The findings demonstrated how ZnSO₄ combined with Na₂SeO₃ treatment reduced stress inhibition and the accumulation of encouraged selenium and isothiocyanates during the growth of broccoli sprouts.

Keywords: Broccoli sprouts; Gene; Isothiocyanates; Proteome; Selenium.