Transcriptome analysis of Euwallacea interjectus reveals differentially expressed unigenes related to developmental stages and egg laying

Jiafeng Hu; Chen Zhao; Jiajin Tan; Shengchang Lai; Yang Zhou; Lulu Dai

doi:10.1016/j.cbd.2023.101100

Transcriptome analysis of Euwallacea interjectus reveals differentially expressed unigenes related to developmental stages and egg laying

Comp Biochem Physiol Part D Genomics Proteomics. 2023 Sep:47:101100. doi: 10.1016/j.cbd.2023.101100. Epub 2023 May 30.

Authors

Jiafeng Hu¹, Chen Zhao¹, Jiajin Tan¹, Shengchang Lai¹, Yang Zhou¹, Lulu Dai²

Affiliations

¹ Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210036, China; College of Forestry, Nanjing Forestry University, Nanjing 210036, China.
² Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210036, China; College of Forestry, Nanjing Forestry University, Nanjing 210036, China. Electronic address: dailulu@njfu.edu.cn.

PMID: 37329642
DOI: 10.1016/j.cbd.2023.101100

Abstract

Euwallacea interjectus (Curculionidae: Scolytinae) is an ambrosia beetle species in its early stages of research. Therefore, studying the related molecular mechanism associated with the development and egg laid is essential. Transcriptome sequencing was used in this study to compare the gene expression of the beetles at different developmental stages and female adults before and after oviposition. A total of 40,047 annotated unigenes were obtained. There were 4225 differentially expressed unigenes (DEUs) from larva to prepupa stage, 3651 DEUs between prepupa and pupa, 1675 DEUs generated from pupa to adult, and 4762 DEUs between females before and after oviposition. The most significant pathway differences between different development stages and before and after oviposition were selected through functional annotation of DEUs between different stages. Among them, there were many pathways related to protein metabolism including: neuroactive ligand-receptor interaction, endoplasmic reticulum and RNA transport. This study provides valuable information on the molecular regulation mechanism of development and the egg laid of E. interjectus.

Keywords: Development; Differentially expressed unigenes; Euwallacea interjectus; Oviposition; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Female
Gene Expression Profiling
Larva
Oviposition
Transcriptome
Weevils* / genetics