Biomass contains different macroconstituents (polysaccharides, lipids, and proteins) with nutritional and functional properties. However, after harvest or processing, stabilization of biomass is necessary to preserve the macroconstituents from degradation by microbial growth and enzymatic reactions. Because these stabilization methods affect the structure of the biomass, extraction of valuable macroconstituents can be impacted. Literature, in general, focuses on either stabilization or extraction, but systematic information on the interlinkage between these processes has rarely been reported. This review summarizes recent research on physical, biological, and chemical stabilization methods on macroconstituent extraction yields and functionalities. Often, freeze drying as a stabilization method resulted in a good extraction yield and functionality, independent of the macroconstituent. Less documented treatments, such as microwave drying, infrared drying, and ultrasound stabilization, result in better yields compared to conventional physical treatments. Biological and chemical treatments were rarely performed but could be promising as stabilization methods before performing an extraction step.
Keywords: extraction; lipids; polysaccharides; proteins; stabilization.