Isolation and genomics of ten novel Shewanella species from mangrove wetland

Guo-Hong Liu; Shang Yang; Manik Prabhu Narsing Rao; Shuang Han; Cheng-Jie Xie; Hend A Alwathnani; Martin Herzberg; Christopher Rensing; Shun-Gui Zhou

doi:10.1099/ijsem.0.005929

Isolation and genomics of ten novel Shewanella species from mangrove wetland

Int J Syst Evol Microbiol. 2023 Jun;73(6). doi: 10.1099/ijsem.0.005929.

Authors

Guo-Hong Liu¹, Shang Yang², Manik Prabhu Narsing Rao³, Shuang Han², Cheng-Jie Xie², Hend A Alwathnani⁴, Martin Herzberg⁵, Christopher Rensing², Shun-Gui Zhou²

Affiliations

¹ Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou City, Fujian Province, 350003, PR China.
² Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province, 350002, PR China.
³ Programa de Doctorado en Ciencias Aplicadas, Universidad Autónoma de Chile, Talca, 3460000, Chile.
⁴ Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
⁵ Department of Microbiology, Martin Luther University Halle-Wittenberg, Halle, Germany.

PMID: 37327059
DOI: 10.1099/ijsem.0.005929

Abstract

Mangrove bacteria largely compose the microbial community of the coastal ecosystem and are directly associated with nutrient cycling. In the present study, 12 Gram-negative and motile strains were isolated from a mangrove wetland in Zhangzhou, China. Pairwise comparisons (based on 16S rRNA gene sequences) and phylogenetic analysis indicated that these 12 strains belong to the genus Shewanella. The 16S rRNA gene sequence similarities among the 12 Shewanella strains and their related type strains ranged from 98.8 to 99.8 %, but they still could not be considered as known species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the 12 strains and their related type strains were below the cut-off values (ANI 95-96% and dDDH 70 %) for prokaryotic species delineation. The DNA G+C contents of the present study strains ranged from 44.4 to 53.8 %. The predominant menaquinone present in all strains was MK-7. The present study strains (except FJAT-53532^T) also contained ubiquinones (Q-8 and Q-7). The polar lipid phosphatidylglycerol and fatty acid iso-C_{15 : 0} was noticed in all strains. Based on phenotypic, chemotaxonomic, phylogenetic and genomic comparisons, we propose that these 12 strains represent 10 novel species within the genus Shewanella, with the names Shewanella psychrotolerans sp. nov. (FJAT-53749^T=GDMCC 1.2398^T=KCTC 82649^T), Shewanella zhangzhouensis sp. nov. (FJAT-52072^T=MCCC 1K05363^T=KCTC 82447^T), Shewanella rhizosphaerae sp. nov. (FJAT-53764^T=GDMCC 1.2349^T=KCTC 82648^T), Shewanella mesophila sp. nov. (FJAT-53870^T=GDMCC 1.2346^T= KCTC 82640^T), Shewanella halotolerans sp. nov. (FJAT-53555^T=GDMCC 1.2344^T=KCTC 82645^T), Shewanella aegiceratis sp. nov. (FJAT-53532^T=GDMCC 1.2343^T=KCTC 82644^T), Shewanella alkalitolerans sp. nov. (FJAT-54031^T=GDMCC 1.2347^T=KCTC 82642^T), Shewanella spartinae sp. nov. (FJAT-53681^T=GDMCC 1.2345^T=KCTC 82641^T), Shewanella acanthi sp. nov. (FJAT-51860^T=GDMCC 1.2342^T=KCTC 82650^T) and Shewanella mangrovisoli sp. nov. (FJAT-51754^T=GDMCC 1.2341^T= KCTC 82647^T).

Keywords: genome-based taxonomy; habitat adaptability; mangrove wetland; metabolic pathways; novel Shewanella species.

MeSH terms

Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Ecosystem
Fatty Acids* / chemistry
Genomics
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Shewanella*
Wetlands

Substances

Fatty Acids
RNA, Ribosomal, 16S
DNA, Bacterial