Frozen Block Tissue Staining for Eye Structure of Zebrafish Embryo

Yujin Lee; Jaewook Yang

doi:10.1007/978-1-0716-3255-0_15

Frozen Block Tissue Staining for Eye Structure of Zebrafish Embryo

Methods Mol Biol. 2023:2678:191-198. doi: 10.1007/978-1-0716-3255-0_15.

Authors

Yujin Lee¹, Jaewook Yang²

Affiliations

¹ Department of Ophthalmology, Inje University Busan Paik Hospital, Busan, South Korea.
² Department of Ophthalmology, Inje University Busan Paik Hospital, Busan, South Korea. jwyang@eyebiokorea.com.

PMID: 37326715
DOI: 10.1007/978-1-0716-3255-0_15

Abstract

Histology is a technique used to study the morphology of cell structures by cutting samples into thin sections. Histological cross-section and staining are the techniques needed to visualize the morphology of cell tissues. A suitable tissue staining experiment was created to observe changes in the retinal layer in zebrafish embryos. Zebrafish have a human-like visual system, retina, and eye structures. Due to the small size of zebrafish and undeveloped bones in the embryonic stage, the resistance through cross-section is inevitably small. Here, we present optimized protocol changes in eye tissue of zebrafish using frozen blocks.

Keywords: Embryo; Eye; Frozen block; Histology; Retina; Tissue; Zebrafish.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Embryo, Nonmammalian
Humans
Retina* / pathology
Staining and Labeling
Zebrafish*