Methods for Ex Vivo AAV Transduction in Rodent and Human Retinal Explants

Satheesh Kumar; Jiang-Hui Wang; Guei-Sheung Liu

doi:10.1007/978-1-0716-3255-0_12

Methods for Ex Vivo AAV Transduction in Rodent and Human Retinal Explants

Methods Mol Biol. 2023:2678:169-175. doi: 10.1007/978-1-0716-3255-0_12.

Authors

Satheesh Kumar^{1

2

3}, Jiang-Hui Wang¹, Guei-Sheung Liu^{4

5

6}

Affiliations

¹ Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC, Australia.
² Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, VIC, Australia.
³ Menzies Institute for Medical Research, University of Tasmania, Hobart, TAS, Australia.
⁴ Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC, Australia. rickliu0817@gmail.com.
⁵ Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, VIC, Australia. rickliu0817@gmail.com.
⁶ Menzies Institute for Medical Research, University of Tasmania, Hobart, TAS, Australia. rickliu0817@gmail.com.

PMID: 37326712
DOI: 10.1007/978-1-0716-3255-0_12

Abstract

Translational research is heavily dependent on animal models, and reliable disease models are essential for the development of novel therapies. Here, we outline the methods for culturing mouse and human retinal explants. In addition, we show efficient adeno-associated virus (AAV) transduction of the mouse retinal explants to aid the study and development of AAV-based therapeutics against ocular diseases.

Keywords: Adeno-associated virus; Retinal explants.

MeSH terms

Animals
Dependovirus* / genetics
Genetic Vectors / genetics
Humans
Mice
Retina
Rodentia*
Transduction, Genetic
Vision, Ocular