Induction of Type 2 Diabetes in Mice to Understand Vascular Changes That Drive Diabetic Retinopathy

Emily Attrill; Stephen M Richards; Renee M Ross; Brad A Sutherland; Dino Premilovac

doi:10.1007/978-1-0716-3255-0_1

Induction of Type 2 Diabetes in Mice to Understand Vascular Changes That Drive Diabetic Retinopathy

Methods Mol Biol. 2023:2678:1-12. doi: 10.1007/978-1-0716-3255-0_1.

Authors

Emily Attrill¹, Stephen M Richards¹, Renee M Ross¹, Brad A Sutherland¹, Dino Premilovac²

Affiliations

¹ Tasmanian School of Medicine, University of Tasmania, Hobart, TAS, Australia.
² Tasmanian School of Medicine, University of Tasmania, Hobart, TAS, Australia. dino.premilovac@utas.edu.au.

PMID: 37326701
DOI: 10.1007/978-1-0716-3255-0_1

Abstract

Diabetic retinopathy is a common complication of type 2 diabetes. Research into this comorbidity is challenging due to the slow progression of pathological changes and the limited transgenic models available to study disease progression and mechanistic changes. Here, we describe a non-transgenic mouse model of accelerated type 2 diabetes using a high-fat diet in combination with streptozotocin delivered via osmotic mini pump. This model, when subjected to fluorescent gelatin vascular casting, can be used to study vascular changes in type 2 diabetic retinopathy.

Keywords: High-fat diet; Insulin resistance; Obesity; Retinopathy; Streptozotocin; Type 2 diabetes; Vascular cast.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diabetes Mellitus, Experimental* / complications
Diabetes Mellitus, Experimental* / pathology
Diabetes Mellitus, Type 2* / complications
Diabetic Retinopathy* / etiology
Diabetic Retinopathy* / pathology
Disease Models, Animal
Mice