Hymenobacter canadensis sp. nov., isolated from freshwater of the pond in Cambridge Bay, Canada

Abstract

A Gram-stain-negative, aerobic, reddish-coloured, rod-shaped and non-motile strain PAMC 29467^T, was isolated from freshwater of the pond in Cambridge Bay, Canada. Strain PAMC 29467^T was closely related to Hymenobacter yonginensis (98.1 % 16S rRNA gene similarity). Genomic relatedness analyses showed that strain PAMC 29467^T is distinguishable from H. yonginensis based on average nucleotide identity (91.3 %) and digital DNA-DNA hybridization values (39.3 %). The major fatty acids (>10 %) of strain PAMC 29467^T were summed feature 3 (C_{16 : 1} ω7c and/or C_{16 : 1} ω6c), C_{15 : 0} iso, C_{16 : 1} ω5c and summed feature 4 (C_{17 : 1} iso l and/or anteiso B). The major respiratory quinone was menaquinone-7. The genomic DNA G+C content was 61.5 mol%. Strain PAMC 29467^T was separated from the type species in the genus Hymenobacter by its distinct phylogenetic position and some physiological characteristics. As a result, a novel species is proposed, with the name Hymenobacter canadensis sp. nov. (type strain, PAMC 29467^T=KCTC 92787^T=JCM 35843^T).

Keywords: Arctic; Hymenobacter canadensis; novel species.