Pectin is identified as an effective delivery material due to its excellent gel-forming ability, low immunogenic properties, biocompatibility, and biodegradability. These excellent properties depend on the preparation method of pectin. In the study, four pectin fractions (named: CAHP30, CAHP40, CAHP50, and CAHP60, respectively) were obtained by different ethanol precipitations (30%, 40%, 50%, and 60%). Physicochemical properties, antioxidant activity, and emulsifying ability of HP were investigated and analyzed. Results showed that the surface structure of pectin was changed by ethanol fractional precipitation, and four fractions were low methoxy pectin. They had different monosaccharide compositions, but all rich in GalA. The Mw/Mn of CAHP30, CAHP40, CAHP50, and CAHP60 were 3.29, 2.57, 2.66, and 2.77, respectively. CAHP30 and CAHP60 had excellent emulsifying ability; moreover, CAHP60 was endowed with additional lipid antioxidant capacity and had the best thermal stability. E-CAHP40 exhibited a property between the entangled network structure. Overall, pectin with specific properties could be obtained by different ethanol concentrations.
Keywords: characterization; ethanol precipitation; hawthorn; pectin.
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