Synergistic effect of carbon nanoparticles with mild salinity for improving chemical composition and antioxidant activities of radish sprouts

Riyadh F Halawani; Hamada AbdElgawad; Fahed A Aloufi; Mansour A Balkhyour; Ahlem Zrig; Abdelrahim H A Hassan

doi:10.3389/fpls.2023.1158031

Synergistic effect of carbon nanoparticles with mild salinity for improving chemical composition and antioxidant activities of radish sprouts

Front Plant Sci. 2023 May 31:14:1158031. doi: 10.3389/fpls.2023.1158031. eCollection 2023.

Authors

Riyadh F Halawani¹, Hamada AbdElgawad², Fahed A Aloufi¹, Mansour A Balkhyour¹, Ahlem Zrig^{3

4}, Abdelrahim H A Hassan^{5

6}

Affiliations

¹ Department of Environment, Faculty of Environmental Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
² Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
³ Higher Institute of Preparatory Studies in Biology and Geology, University of Carthage, Tunis, Tunisia.
⁴ Laboratory of Engineering Processes and Industrial Systems, Chemical Engineering Department, National School of Engineers of Gabes, University of Gabes, Gabès, Tunisia.
⁵ School of Biotechnology, Nile University, Giza, Egypt.
⁶ Department of Food Safety and Technology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.

Abstract

The demand for healthy foods with high functional value has progressively increased. Carbon nanoparticles (CNPs) have a promising application in agriculture including the enhancement of plant growth. However, there are few studies on the interactive effects of CNPs and mild salinity on radish seed sprouting. To this end, the effect of radish seed priming with 80mM CNPs on biomass, anthocyanin, proline and polyamine metabolism, and antioxidant defense system under mild salinity growth condition (25 mM NaCl). The results indicated that seed nanopriming with CNPs along with mild salinity stress enhanced radish seed sprouting and its antioxidant capacity. Priming boosted the antioxidant capacity by increasing antioxidant metabolites such as (polyphenols, flavonoids, polyamines, anthocyanin, and proline). To understand the bases of these increases, precursors and key biosynthetic enzymes of anthocyanin [phenylalanine, cinnamic acid, coumaric acid, naringenin, phenylalanine ammonia lyase, chalcone synthase (CHS), cinnamate-4-hydroxylase (C4H) and 4-coumarate: CoA ligase (4CL)], proline [pyrroline-5-carboxylate synthase (P5CS), proline dehydrogenase (PRODH), Sucrose, Sucrose P synthase, invertase) and polyamines [putrescine, spermine, spermidine, total polyamines, arginine decarboxylase, orinthnine decarboxylase, S-adenosyl-L-methionine decarboxylase, spermidine synthase, spermine synthase] were analyzed. In conclusion, seed priming with CNPs has the potential to further stimulate mild salinity-induced bioactive compound accumulation in radish sprouts.

Keywords: anthocyanin; carbon nanoparticles; mild salinity; polyamines; proline; radish sprouts.

Grants and funding

This research work was funded by Institutional Fund Projects under grant no. (IFPIP: 612-155-1443). The authors gratefully acknowledge technical and financial support provided by the Ministry of Education and King Abdulaziz University, DSR, Jeddah, Saudi Arabia.