Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress

Su Yang; Jialuo Chen; Yonghe Ding; Qian Huang; Guangna Chen; Zaid Ulhassan; Ji'an Wei; Jian Wang

doi:10.3389/fpls.2023.1197781

Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress

Front Plant Sci. 2023 May 31:14:1197781. doi: 10.3389/fpls.2023.1197781. eCollection 2023.

Authors

Su Yang^{1

2}, Jialuo Chen¹, Yonghe Ding¹, Qian Huang², Guangna Chen¹, Zaid Ulhassan², Ji'an Wei³, Jian Wang⁴

Affiliations

¹ Key Laboratory of Specialty Agri-products Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China.
² Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, China.
³ Mizuda Group Co., Ltd., Huzhou, Zhejiang, China.
⁴ Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Abstract

The Brassica napus (B. napus) LOR (Lurp-One-Related) gene family is a little-known gene family characterized by a conserved LOR domain in the proteins. Limited research in Arabidopsis showed that LOR family members played important roles in Hyaloperonospora parasitica (Hpa) defense. Nevertheless, there is a paucity of research investigating the role of the LOR gene family towards their responses to abiotic stresses and hormone treatments. This study encompassed a comprehensive survey of 56 LOR genes in B. napus, which is a prominent oilseed crop that holds substantial economic significance in China, Europe, and North America. Additionally, the study evaluated the expression profiles of these genes in response to salinity and ABA stress. Phylogenetic analysis showed that 56 BnLORs could be divided into 3 subgroups (8 clades) with uneven distribution on 19 chromosomes. 37 out of 56 BnLOR members have experienced segmental duplication and 5 of them have undergone tandem repeats events with strong evidence of purifying selection. Cis-regulatory elements (CREs) analysis indicated that BnLORs involved in process such as light response, hormone response, low temperature response, heat stress response, and dehydration response. The expression pattern of BnLOR family members revealed tissue specificity. RNA-Seq and qRT-PCR were used to validate BnLOR gene expression under temperature, salinity and ABA stress, revealing that most BnLORs showed inducibility. This study enhanced our comprehension of the B. napus LOR gene family and could provide valuable information for identifying and selecting genes for stress resistant breeding.

Keywords: Brassica napus; LOR gene family; abiotic stress; expression profiles; phylogenetic tree.

Grants and funding

This research was supported by the National Natural Science Foundation of China (32200251) and the Science and Technology Department of Zhejiang Province (2021C02064-2).