Visible light photocrosslinking of sugar beet pectin for 3D bioprinting applications

Wildan Mubarok; Kelum Chamara Manoj Lakmal Elvitigala; Takashi Kotani; Shinji Sakai

doi:10.1016/j.carbpol.2023.121026

Visible light photocrosslinking of sugar beet pectin for 3D bioprinting applications

Carbohydr Polym. 2023 Sep 15:316:121026. doi: 10.1016/j.carbpol.2023.121026. Epub 2023 May 15.

Authors

Wildan Mubarok¹, Kelum Chamara Manoj Lakmal Elvitigala², Takashi Kotani³, Shinji Sakai⁴

Affiliations

¹ Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. Electronic address: wildanmubarok@cheng.es.osaka-u.ac.jp.
² Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. Electronic address: kelum@cheng.es.osaka-u.ac.jp.
³ Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. Electronic address: t.kotani@cheng.es.osaka-u.ac.jp.
⁴ Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. Electronic address: sakai@cheng.es.osaka-u.ac.jp.

PMID: 37321724
DOI: 10.1016/j.carbpol.2023.121026

Abstract

Herein, we report the hydrogelation of sugar beet pectin (SBP) via visible light-mediated photocrosslinking and its applications in extrusion-based 3D bioprinting. Rapid hydrogelation (<15 s) was achieved by applying 405 nm visible light to an SBP solution in the presence of tris(bipyridine)ruthenium(II) chloride hexahydrate ([Ru(bpy)₃]²⁺) and sodium persulfate (SPS). The mechanical properties of the hydrogel could be tuned by controlling the visible light irradiation time and concentrations of SBP, [Ru(bpy)₃]²⁺, and SPS. High-fidelity 3D hydrogel constructs were fabricated by extruding inks containing 3.0 wt% SBP, 1.0 mM [Ru(bpy)₃]²⁺, and 1.0 mM SPS. Human hepatoblastoma (HepG2) cells encapsulated in SBP hydrogels remained viable and metabolically active after 14 d of culture. Overall, this study demonstrates the feasibility of applying SBP and a visible light-mediated photocrosslinking system to the 3D bioprinting of cell-laden constructs for tissue engineering applications.

Keywords: 3D bioprinting; Photocrosslinking; Sodium persulfate; Sugar beet pectin; Tissue engineering.

MeSH terms

Beta vulgaris*
Bioprinting*
Humans
Hydrogels
Light
Pectins
Sugars

Substances

Pectins
Hydrogels
sodium persulfate
Sugars