Processing and characterization of egg shell derived nano-hydroxyapatite synthetic bone for Orthopaedic and Arthroscopy implants and substitutes in dentistry

B Y Santosh Kumar; G C Mohan Kumar; Kiran Shahapurkar; Vineet Tirth; Ali Algahtani; Tawfiq Al-Mughanam; Abdulaziz H Alghtani; H C Ananda Murthy

doi:10.1016/j.jmbbm.2023.105963

Processing and characterization of egg shell derived nano-hydroxyapatite synthetic bone for Orthopaedic and Arthroscopy implants and substitutes in dentistry

J Mech Behav Biomed Mater. 2023 Aug:144:105963. doi: 10.1016/j.jmbbm.2023.105963. Epub 2023 Jun 7.

Authors

B Y Santosh Kumar¹, G C Mohan Kumar², Kiran Shahapurkar³, Vineet Tirth⁴, Ali Algahtani⁴, Tawfiq Al-Mughanam⁵, Abdulaziz H Alghtani⁶, H C Ananda Murthy⁷

Affiliations

¹ Sironix Division - Arthroscopy & Sports Medicine, Healthium Medtech, Bangalore, 560058, India. Electronic address: santosh.ait666@gmail.com.
² Polymer Composites Laboratory, Department of Mechanical Engineering, National Institute of Technology Karnataka, Surathkal, Mangalore, 575 025, India.
³ Department of Mechanical Engineering, School of Mechanical, Chemical and Materials Engineering, Adama Science and Technology University, Adama, 1888, Ethiopia.
⁴ Mechanical Engineering Department, College of Engineering, King Khalid University, Abha, 61411, Asir, Saudi Arabia; Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha, 61413, P.O. Box No. 9004, Asir, Saudi Arabia.
⁵ Department of Mechanical Engineering, College of Engineering, King Faisal University, P.O. Box 380, Al-Ahsa, 31982, Saudi Arabia.
⁶ Department of Mechanical Engineering, College of Engineering, Taif University, P.O. Box 11099, Taif, 21944, Kingdom of Saudi Arabia.
⁷ Department of Applied Chemistry, School of Applied Natural Science, Adama Science and Technology University, P.O. Box 1888, Adama, Ethiopia; Department of Prosthodontics, Saveetha Dental College & Hospital, Saveetha Institute of Medical and Technical Science (SIMAT), Saveetha University, Chennai, 600077, Tamil Nadu, India. Electronic address: anandkps350@gmail.com.

PMID: 37320892
DOI: 10.1016/j.jmbbm.2023.105963

Abstract

The present work is focused on the nano-Hydroxyapatite (nHAp) synthesis with two different Indian breed Aseel and Kadaknath eggshells. The alloplast implants were developed through the foam replica method with polyurethane 45-PPI as a porous template. The synthesized nHAp was characterized by Field Emission Scanning Electron Microscopy (FE-SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The FE-SEM images of the nHAp showed the one dimensional clustered nanoparticles and the X-ray diffraction spectrum confirms that the major phase was hydroxyapatite with a small trace of β-tricalcium phosphate. The maximum compression strength of the sample was 5.49 ± 0.12 MPa which is in the range of the compression strength of human trabecular bone. The thermal and degradability studies results confirmed that these are highly stable and provides necessary a resorption needed for new bone tissue formation. Besides, the antimicrobial activity against tested human microbiome are satisfactory and the cell viability towards MG 63 human osteoblast-like cells provides a potential pathway for developing the nHAp implants for bone tissue engineering.

Keywords: Egg shell; Nanohydroxyapatite; Trabecular bone tissue.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arthroscopy
Bone and Bones
Dentistry
Durapatite* / chemistry
Egg Shell
Humans
Orthopedics*
Spectroscopy, Fourier Transform Infrared
Tissue Engineering / methods
Tissue Scaffolds / chemistry
X-Ray Diffraction

Substances

Durapatite
N-hydroxy-2-aminopyrene