Genome Sequence of a Clostridioides difficile Strain Isolated from Feces from a Patient in Southern Thailand

Thunchanok Yaikhan; Komwit Surachat; Chonticha Romyasamit; Pawarisa Luenglusontigit; Arnon Chukamnerd; Phanvasri Saengsuwan; Monwadee Wonglapsuwan; Kamonnut Singkhamanan

doi:10.1128/mra.00455-23

Genome Sequence of a Clostridioides difficile Strain Isolated from Feces from a Patient in Southern Thailand

Microbiol Resour Announc. 2023 Jul 18;12(7):e0045523. doi: 10.1128/mra.00455-23. Epub 2023 Jun 15.

Authors

Thunchanok Yaikhan¹, Komwit Surachat^{1

2}, Chonticha Romyasamit^{1

3}, Pawarisa Luenglusontigit¹, Arnon Chukamnerd⁴, Phanvasri Saengsuwan¹, Monwadee Wonglapsuwan⁵, Kamonnut Singkhamanan¹

Affiliations

¹ Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
² Translational Medicine Research Center, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
³ Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand.
⁴ Division of Infectious Diseases, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
⁵ Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla, Thailand.

Abstract

Clostridioides difficile is a Gram-positive, obligate anaerobic, toxin-producing bacillus that is linked to antibiotic-associated diarrhea. Here, we report the whole-genome sequence of a C. difficile strain isolated from stool from a patient, using next-generation sequencing (MGISEG-2000). De novo assembly revealed a genome length of 4,208,266 bp. Multilocus sequence typing (MLST) results showed that the isolate belonged to sequence type 23 (ST23).