Protocol for purification of cells in their native state using reversible aptamer-antidote pairs

Abstract

Cell isolation from complex mixtures is a key step in many clinical and research applications, but standard isolation methods may affect the cell's biology and are difficult to reverse. Here, we present a method to isolate and restore cells to their native state using an aptamer that binds epidermal growth factor receptor (EGFR+)cells and a complementary antisense oligonucleotide to reverse binding. For complete details on the use and execution of this protocol, please refer to Gray et al.¹.

Keywords: Biotechnology and bioengineering; Cell Biology; Cell isolation; Molecular/Chemical Probes.